Description
We have used a simple and inexpensive, self-built particle acceleration apparatus for direct delivery of DNA to cultured cells of warm-season grasses. High levels of transient expression of the b-glucuronidase gene were obtained following bombardment of embryogenic suspension cells and calli of bahiagrass (Paspalum notatum Flügge) and dallisgrass (Paspalum dilatatum Poir). Furthermore, stable transformed calli of both species have been obtained using this simple particle gun.
Citation
Yuge, C; Akashi, R; and Kawamura, O, "Improvement of Forage Quality by Means of Biotechnology: Stable Transformation of Warm-Season Grasses by Particle Bombardment" (2024). IGC Proceedings (1993-2023). 6.
https://uknowledge.uky.edu/igc/1997/session4/6
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Improvement of Forage Quality by Means of Biotechnology: Stable Transformation of Warm-Season Grasses by Particle Bombardment
We have used a simple and inexpensive, self-built particle acceleration apparatus for direct delivery of DNA to cultured cells of warm-season grasses. High levels of transient expression of the b-glucuronidase gene were obtained following bombardment of embryogenic suspension cells and calli of bahiagrass (Paspalum notatum Flügge) and dallisgrass (Paspalum dilatatum Poir). Furthermore, stable transformed calli of both species have been obtained using this simple particle gun.
