Description
We have developed a rapid and reproducible transformation system for bird’s-foot trefoil (Lotus corniculatus L.) by using Agrobacterium-mediated T-DNA transfer and the incorporation of the antisense gene for cinnamyl alcohol dehydrogenase (CAD) from Aralia cordata into Lotus for lignin reduction. The presence of the transferred antisense gene in regenerated plants has been confirmed by PCR analysis.
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Transformation in Lotus Corniculatus: Towards Low-Lignin Pasture Through Antisense RNA
We have developed a rapid and reproducible transformation system for bird’s-foot trefoil (Lotus corniculatus L.) by using Agrobacterium-mediated T-DNA transfer and the incorporation of the antisense gene for cinnamyl alcohol dehydrogenase (CAD) from Aralia cordata into Lotus for lignin reduction. The presence of the transferred antisense gene in regenerated plants has been confirmed by PCR analysis.
