Publication Date
1997
Location
Manitoba and Saskatchewan
Description
We have developed a rapid and reproducible transformation system for bird’s-foot trefoil (Lotus corniculatus L.) by using Agrobacterium-mediated T-DNA transfer and the incorporation of the antisense gene for cinnamyl alcohol dehydrogenase (CAD) from Aralia cordata into Lotus for lignin reduction. The presence of the transferred antisense gene in regenerated plants has been confirmed by PCR analysis.
Citation
Akashi, R; Uchiyama, T; Sakatani, Y; and Kawamura, O, "Transformation in Lotus Corniculatus: Towards Low-Lignin Pasture Through Antisense RNA" (1997). IGC Proceedings (1985-2023). 5.
(URL: https://uknowledge.uky.edu/igc/1997/session4/5)
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Transformation in Lotus Corniculatus: Towards Low-Lignin Pasture Through Antisense RNA
Manitoba and Saskatchewan
We have developed a rapid and reproducible transformation system for bird’s-foot trefoil (Lotus corniculatus L.) by using Agrobacterium-mediated T-DNA transfer and the incorporation of the antisense gene for cinnamyl alcohol dehydrogenase (CAD) from Aralia cordata into Lotus for lignin reduction. The presence of the transferred antisense gene in regenerated plants has been confirmed by PCR analysis.
