Abstract
Currently available diagnostic assays for leptospirosis cannot differentiate vaccine from infection serum antibody. Several leptospiral proteins that are upregulated during infection have been described, but their utility as a diagnostic marker is still unclear. In this study, we undertook a lipidomics approach to determine if there are any differences in the serum lipid profiles of horses naturally infected with pathogenic Leptospira spp. and horses vaccinated against a commercially available bacterin. Utilizing a high-resolution mass spectrometry serum lipidomics analytical platform, we demonstrate that cyclic phosphatidic acids, diacylglycerols, and hydroperoxide oxidation products of choline plasmalogens are elevated in the serum of naturally infected as well as vaccinated horses. Other lipids of interest were triacylglycerols that were only elevated in the serum of infected horses and sphingomyelins that were increased only in the serum of vaccinated horses. This is the first report looking at the equine serum lipidome during leptospiral infection and vaccination.
Document Type
Article
Publication Date
2-23-2018
Digital Object Identifier (DOI)
https://doi.org/10.1371/journal.pone.0193424
Funding Information
Funds for this work were provided by LMU-College of Veterinary Medicine’s internal grant program.
Related Content
Data Availability: Data are available in a Supplementary Table.
Repository Citation
Wood, Paul L.; Steinman, Margaret; Erol, Erdal; Carter, Craig; Christmann, Undine; and Verma, Ashutosh, "Lipidomic Analysis of Immune Activation in Equine Leptospirosis and Leptospira-Vaccinated Horses" (2018). Veterinary Diagnostic Laboratory Faculty Publications. 4.
https://uknowledge.uky.edu/vdl_facpub/4
S1 Table.
Notes/Citation Information
Published in PLOS ONE, v. 13, no. 2, e0193424, p. 1-14.
Copyright: © 2018 Wood et al.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.