Description
Condensed tannins (CT) from sainfoin have a high capacity to inhibit proteolysis. The objective of the present study was to investigate the effects of CT (following supplementation of deactivated CT with polyethylene glycol [PEG]) on the metabolome during sainfoin ensiling. In total, 510 metabolites were identified after 60 d of sainfoin ensiling, with 33 metabolites were annotated in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Among those metabolites, phospholipids were the most abundant (72.7% of total 33 metabolites). In addition, 10 up-regulated and 23 down-regulated metabolites, respectively, were identified in the PEG treated group when compared with the control group, after 60 d of ensiling (p < 0.05). Pediococcus (correlated with 20 metabolites, R2 > 0.88, p< 0.05) and Lactobacillus (correlated with 16 metabolites, R2 > 0.88, p < 0.05) were the bacteria most correlated with metabolites. The results suggest antagonistic effects between Lactobacillus and Pediococcus occur during ensiling. The proteolysis decreased partly due to CT inhibiting Pediococcus activity during ensiling, with Pediococcus being significantly and positively correlated with dopamine after 60 d of ensiling (R2=0.8857, p < 0.05).
DOI
https://doi.org/10.13023/rvhs-1b84
Citation
Huang, Rongzheng; Ma, Chunhui; Zhang, Fanfan; and Wang, Xuzhe, "Effects of Intrinsic Tannins on Metabolome During Sainfoin Ensiling" (2024). IGC Proceedings (1993-2023). 83.
https://uknowledge.uky.edu/igc/XXV_IGC_2023/Utilization/83
Included in
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Effects of Intrinsic Tannins on Metabolome During Sainfoin Ensiling
Condensed tannins (CT) from sainfoin have a high capacity to inhibit proteolysis. The objective of the present study was to investigate the effects of CT (following supplementation of deactivated CT with polyethylene glycol [PEG]) on the metabolome during sainfoin ensiling. In total, 510 metabolites were identified after 60 d of sainfoin ensiling, with 33 metabolites were annotated in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Among those metabolites, phospholipids were the most abundant (72.7% of total 33 metabolites). In addition, 10 up-regulated and 23 down-regulated metabolites, respectively, were identified in the PEG treated group when compared with the control group, after 60 d of ensiling (p < 0.05). Pediococcus (correlated with 20 metabolites, R2 > 0.88, p< 0.05) and Lactobacillus (correlated with 16 metabolites, R2 > 0.88, p < 0.05) were the bacteria most correlated with metabolites. The results suggest antagonistic effects between Lactobacillus and Pediococcus occur during ensiling. The proteolysis decreased partly due to CT inhibiting Pediococcus activity during ensiling, with Pediococcus being significantly and positively correlated with dopamine after 60 d of ensiling (R2=0.8857, p < 0.05).
