Description
Interest in ‘AU-Grazer’ sericea lespedeza (Lespedeza cuneata) as an anti-parasitic bioactive forage has been growing in the United States, but the concentration and bioactivity of tannins and other phenolics from additional accessions of L. cuneata, as well as other Lespedeza species, have not been evaluated. A study was completed to determine the concentration of extractable condensed tannins (ECT), total CT (TCT), total phenolics (TP), and protein-precipitable phenolics (PPP) of 32 accessions of L. cuneata and 16 additional Lespedeza species from a germplasm collection. The plants were established in small pots in a greenhouse and then transplanted into small field plots at the Fort Valley State University Agricultural Research Station in Georgia. Once established in the field, samples from each plot were collected, freeze-dried, and ground for analysis. The ECT and TCT for L. cuneata accessions averaged 6.6 ± 1.4% and 9.1 ± 1.8%, respectively, while TP were 114.8 ± 33.2 mg/g plant material and PPP averaged 81.5 ± 25.3 mg binding CT/g plant material. For other Lespedeza species, ECT ranged from 3.7 ± 1.3 (L. striate) to 8.8 ± 1.3 (L. frutescens) and TCT from 6.0 ± 0.5 (L. japonica) to 10.8 ± 1.3 (L. frutescens). Total phenolics ranged from 45.3 ± 24.0 (L. striate) to 185.8 ± 43.9 (L. virgata), and PPP from 22.1 ± 71 (L. tomentosa) to 89.0 ± 23.6 (L. virginica). All Lespedeza species evaluated in this study had relatively high levels of CT, with several species as high or higher in TP and PPP (bioactivity) than L. cuneata, suggesting potential increased use of these plants as nutraceutical forages for animals.
DOI
https://doi.org/10.13023/ek8f-dv05
Citation
Terrill, Thomas H.; Smith, V.; Morning, B.; Courson, E. M.; Muir, James P.; Cherry, N. M.; and Morris, J. B., "Concentration and Bioactivity of Condensed Tannins and Total Phenolics of Lespedeza Species From a Germplasm Collection" (2023). IGC Proceedings (1993-2023). 23.
https://uknowledge.uky.edu/igc/XXV_IGC_2023/Utilization/23
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Concentration and Bioactivity of Condensed Tannins and Total Phenolics of Lespedeza Species From a Germplasm Collection
Interest in ‘AU-Grazer’ sericea lespedeza (Lespedeza cuneata) as an anti-parasitic bioactive forage has been growing in the United States, but the concentration and bioactivity of tannins and other phenolics from additional accessions of L. cuneata, as well as other Lespedeza species, have not been evaluated. A study was completed to determine the concentration of extractable condensed tannins (ECT), total CT (TCT), total phenolics (TP), and protein-precipitable phenolics (PPP) of 32 accessions of L. cuneata and 16 additional Lespedeza species from a germplasm collection. The plants were established in small pots in a greenhouse and then transplanted into small field plots at the Fort Valley State University Agricultural Research Station in Georgia. Once established in the field, samples from each plot were collected, freeze-dried, and ground for analysis. The ECT and TCT for L. cuneata accessions averaged 6.6 ± 1.4% and 9.1 ± 1.8%, respectively, while TP were 114.8 ± 33.2 mg/g plant material and PPP averaged 81.5 ± 25.3 mg binding CT/g plant material. For other Lespedeza species, ECT ranged from 3.7 ± 1.3 (L. striate) to 8.8 ± 1.3 (L. frutescens) and TCT from 6.0 ± 0.5 (L. japonica) to 10.8 ± 1.3 (L. frutescens). Total phenolics ranged from 45.3 ± 24.0 (L. striate) to 185.8 ± 43.9 (L. virgata), and PPP from 22.1 ± 71 (L. tomentosa) to 89.0 ± 23.6 (L. virginica). All Lespedeza species evaluated in this study had relatively high levels of CT, with several species as high or higher in TP and PPP (bioactivity) than L. cuneata, suggesting potential increased use of these plants as nutraceutical forages for animals.
