Theme 2: Forage--Oral Sessions

Description

Red clover is a valuable legume in the world. Among the biotic factors limiting persistence of the species in Chile is the root borer Hylastinus obscurus (Marsham). Our previous studies have shown a negative effect of formononetin content of the plant on the root borer fitness The purpose of this research was to assess the relationship between water availability and formononetin concentration. Two independent growth chamber experiments were established at Carillanca Research Center, INIA-Chile in order to study the effect of water availability on formononetin concentration in the aerial and root parts of the plants. In experiment I, three levels of water applied were studied: T1: non-stressed, maintained between 100 and 60% of the readily available soil water (RAW); T2: 60 and 30% of RAW; T3: < 30% of RAW. Experiment II consisted of four water levels: T1: 100-80% RAW; T2: 80-60% RAW; T3: 60-40% of RAW; T4: < 40% of RAW. In both experiments a complete randomized design was used. For sampling, plants were dug up from the pots and cut at the crown to separate the aerial part from the crown and roots. Formononetin concentration was evaluated by extracting with a methanol solution and relative quantifications based on HPLC–MS. Different water available levels were studied. Formononetin was much higher in the aerial part compared to roots. There were no significant effects of readily available soil water (RAW) over formononetin concentration.

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Formononetin of Red Clover (Trifolium pratense L.) as Affected by Water Availability

Red clover is a valuable legume in the world. Among the biotic factors limiting persistence of the species in Chile is the root borer Hylastinus obscurus (Marsham). Our previous studies have shown a negative effect of formononetin content of the plant on the root borer fitness The purpose of this research was to assess the relationship between water availability and formononetin concentration. Two independent growth chamber experiments were established at Carillanca Research Center, INIA-Chile in order to study the effect of water availability on formononetin concentration in the aerial and root parts of the plants. In experiment I, three levels of water applied were studied: T1: non-stressed, maintained between 100 and 60% of the readily available soil water (RAW); T2: 60 and 30% of RAW; T3: < 30% of RAW. Experiment II consisted of four water levels: T1: 100-80% RAW; T2: 80-60% RAW; T3: 60-40% of RAW; T4: < 40% of RAW. In both experiments a complete randomized design was used. For sampling, plants were dug up from the pots and cut at the crown to separate the aerial part from the crown and roots. Formononetin concentration was evaluated by extracting with a methanol solution and relative quantifications based on HPLC–MS. Different water available levels were studied. Formononetin was much higher in the aerial part compared to roots. There were no significant effects of readily available soil water (RAW) over formononetin concentration.