Theme 2: Forage--Oral Sessions

Description

Orchardgrass (Dactylis glomerata L.) is an important forage grass for cultivating livestock worldwide. Heading date is a surrogate measure of flowering time and is strongly correlated with the yield and quality of forage grasses. Here, we identified 210 flowering-related genes in orchardgrass by BLASTP. In addition, our integrated analysis of transcriptome, quantitative trait locus (QTL), and bulked segregant analysis (BSA) provided insights into the genetic network regulating flowering time. The results revealed that four main candidate genes may participate in flowering time control, including one FT-like gene and three MADS-box genes. Expression investigation between the early-flowering and late-flowering phenotype of orchardgrass indicated gene DG6G02970.1 was the only significantly differentially expressed gene, which encodes the MADS-box gene AGL61-like. Three nonsynonymous SNPs were identified in the AGL61-like gene, resulting in changes from alanine to valine, alanine to threonine and glycine to valine, most likely to cause differentially expression pattern in early-flowering and late-flowering phenotype of orchardgrass. Weighted gene co-expression network analysis (WGCNA) revealed that AGL61-like is associated with a set of 114 genes relevant to carbohydrate metabolic, oxidative stress response and glycolysis/gluconeogenesis process. These results provided useful clues for flowering time regulation in molecular breeding of forage grasses.

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Identification and Analysis of Flowering Time Candidate Genes of Dactylis glomerata L.

Orchardgrass (Dactylis glomerata L.) is an important forage grass for cultivating livestock worldwide. Heading date is a surrogate measure of flowering time and is strongly correlated with the yield and quality of forage grasses. Here, we identified 210 flowering-related genes in orchardgrass by BLASTP. In addition, our integrated analysis of transcriptome, quantitative trait locus (QTL), and bulked segregant analysis (BSA) provided insights into the genetic network regulating flowering time. The results revealed that four main candidate genes may participate in flowering time control, including one FT-like gene and three MADS-box genes. Expression investigation between the early-flowering and late-flowering phenotype of orchardgrass indicated gene DG6G02970.1 was the only significantly differentially expressed gene, which encodes the MADS-box gene AGL61-like. Three nonsynonymous SNPs were identified in the AGL61-like gene, resulting in changes from alanine to valine, alanine to threonine and glycine to valine, most likely to cause differentially expression pattern in early-flowering and late-flowering phenotype of orchardgrass. Weighted gene co-expression network analysis (WGCNA) revealed that AGL61-like is associated with a set of 114 genes relevant to carbohydrate metabolic, oxidative stress response and glycolysis/gluconeogenesis process. These results provided useful clues for flowering time regulation in molecular breeding of forage grasses.