Theme 2-2: Forage Production and Utilization--Poster Sessions
Description
Lablab (Lablab purpureus L.) is an important annual multi-purpose legume used as a vegetable for human consumption, as forage for livestock, and as green manure and a cover crop to improve soil fertility. It has a high feed value with good digestibility and high crude protein content. The International Livestock Research Institute (ILRI) forage genebank holds a diverse set of 340 lablab accessions collected from different regions of the world. A total of 1,843 plants from 142 lablab accessions (1 to 29 plants per accession genotyped individually) were genotyped by the genotyping-by-sequencing (GBS) method of the DArTseq platform. The genotyping produced a total of 38,824 and 64,793 genome-wide single nucleotide polymorphism (SNP) and SilicoDArT high-density markers, respectively. The short sequence reads corresponding to the markers were mapped on the mungbean (Vigna radiata) reference genome, with approximately 37% of the SNPs and 26 % of the SilicoDArTs able to be mapped. A subset of 1,000 robust markers was filtered by different criteria and used for the diversity analysis. Clustering analysis using the discriminant analysis of principal components (DAPC) detected five major groups, each with further subgroups. Analysis of molecular variance (AMOVA) showed a highly significant (P < 0.00001) variation, explaining more than 73 % of the variance among the accessions. A significant variation (P < 0.005) was also observed among plants within accessions, which explained about 27 % of the variation. The results of this study provide a useful guide for the management and rationalization of activities of the lablab germplasm collection at the ILRI genebank. The substantial genetic diversity observed in the collection reveals the potential of the population for further genetic studies.
Included in
Genetic Diversity among and within Accessions of a Lablab (Lablab purpureus) Collection Maintained in the ILRI Forage Genebank
Lablab (Lablab purpureus L.) is an important annual multi-purpose legume used as a vegetable for human consumption, as forage for livestock, and as green manure and a cover crop to improve soil fertility. It has a high feed value with good digestibility and high crude protein content. The International Livestock Research Institute (ILRI) forage genebank holds a diverse set of 340 lablab accessions collected from different regions of the world. A total of 1,843 plants from 142 lablab accessions (1 to 29 plants per accession genotyped individually) were genotyped by the genotyping-by-sequencing (GBS) method of the DArTseq platform. The genotyping produced a total of 38,824 and 64,793 genome-wide single nucleotide polymorphism (SNP) and SilicoDArT high-density markers, respectively. The short sequence reads corresponding to the markers were mapped on the mungbean (Vigna radiata) reference genome, with approximately 37% of the SNPs and 26 % of the SilicoDArTs able to be mapped. A subset of 1,000 robust markers was filtered by different criteria and used for the diversity analysis. Clustering analysis using the discriminant analysis of principal components (DAPC) detected five major groups, each with further subgroups. Analysis of molecular variance (AMOVA) showed a highly significant (P < 0.00001) variation, explaining more than 73 % of the variance among the accessions. A significant variation (P < 0.005) was also observed among plants within accessions, which explained about 27 % of the variation. The results of this study provide a useful guide for the management and rationalization of activities of the lablab germplasm collection at the ILRI genebank. The substantial genetic diversity observed in the collection reveals the potential of the population for further genetic studies.
