Theme 2-2: Forage Production and Utilization--Poster Sessions

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In this study, the identity of rumen bacterial community and their taxonomic classification in Zebu heifers (n=24) fed 4 different diets (range grasses, Bracharia MulatoII, Azolla and Cassava leaf meal) was surveyed using metagenomics sequencing of the 16SrRNA gene. Rumen liquor samples were collected from the heifers from which a total of 192 DNA samples were amplified and the resulting 16S rRNA sequences compared to the existing sequences at the National Centre for Biotechnology Information (NCBI) BLAST database through the MetagenAssist . Bioinformatics analyses indicated that 17 operational taxonomic units (OTUs) were present at the phylum level. Of these, 43.3% were affiliated to the phylum Firmicutes, 27.2% Bacteroidetes, 22.8% Proteobacteria and 1.7% Euryarchaeota. The remaining were Cyanobacteria (1.4%), Chloroflexi (1%) while Actinobacteria, Verrucomicrobia, Spirochaetes, Tenericutes, Planctomycetes, Elusimicrobia, Lentisphaerae, Armatimonadetes, Fibrobacteres, Synergistetes and Arthropoda were all below 1%. Both Time and Diet had significant effect on the abundance of microbes but did not affect their diversity. Different diets therefore can affect the abundance of rumen microbiome and eventually the performance of animals.

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Diversity and Taxonomic Identity of Rumen Bacterial Community in Cattle Fed Different Diets

In this study, the identity of rumen bacterial community and their taxonomic classification in Zebu heifers (n=24) fed 4 different diets (range grasses, Bracharia MulatoII, Azolla and Cassava leaf meal) was surveyed using metagenomics sequencing of the 16SrRNA gene. Rumen liquor samples were collected from the heifers from which a total of 192 DNA samples were amplified and the resulting 16S rRNA sequences compared to the existing sequences at the National Centre for Biotechnology Information (NCBI) BLAST database through the MetagenAssist . Bioinformatics analyses indicated that 17 operational taxonomic units (OTUs) were present at the phylum level. Of these, 43.3% were affiliated to the phylum Firmicutes, 27.2% Bacteroidetes, 22.8% Proteobacteria and 1.7% Euryarchaeota. The remaining were Cyanobacteria (1.4%), Chloroflexi (1%) while Actinobacteria, Verrucomicrobia, Spirochaetes, Tenericutes, Planctomycetes, Elusimicrobia, Lentisphaerae, Armatimonadetes, Fibrobacteres, Synergistetes and Arthropoda were all below 1%. Both Time and Diet had significant effect on the abundance of microbes but did not affect their diversity. Different diets therefore can affect the abundance of rumen microbiome and eventually the performance of animals.