Track 4-1-1: Plant Genetic Resources, Collection, Conversation, Evaluation and IPR Issues

Description

Switchgrass (Panicum virgatum L.) is an important forage and biomass species for many parts of the USA. Switchgrass can be of several ploidies. Octoploid cultivars are most often used in forage and conservation settings, while the tetraploid cultivars are mostly targeted for bioenergy end-uses, due to their higher biomass yields. Switchgrass populations also occur as upland and lowland ecotypes, and constitute different heterotic groups. Switchgrass is mostly an obligate outcrosser resulting in substantial genotypic and phenotypic variation within populations. In the last ~15 years, significant resources have been dedicated to both breeding and understanding the genomic makeup of this plant, with a focus on bioenergy. This investment has resulted in the development of elite lines as well as a considerable increase in available genetic, physiological, and biomass-related information. The United States Department of Agriculture-Agricultural Research Service has been a major player in these developments (Mitchell and Schmer, 2012; Vogel et al., 2011).

With significant improvements in DNA-sequencing technologies (High Throughput Sequencing, HTS), it has become possible to undertake large-scale analysis of both the genomic and functional genomic components of switchgrass. One such undertaking by the United States Department of Energy-Joint Genomics Institute has provided a draft assembly and annotation of the switchgrass genome (www.phytozome.org). This remarkable resource has permitted a complete utilization of HTS to analyze gene expression using RNA-Seq and related bioinformatic pipelines. Large-scale studies that are performed using field-grown plants and populations with well-characterized phenotypic traits, it increases the likelihood of discovering molecular events that underpin phenomena of interest. Even though lowland tetraploid cultivars have higher biomass yields than upland tetraploid cultivars, they can suffer significant winter-kill in more northern locations (Central Great Plains of the USA). Winter-kill is associated with the loss of rhizomes and other perenniating structures resulting in a complete or partial loss of tillering ability in the following seasons. Partial attrition of tiller production serves to limit new rhizome growth in successive years. One or more cycles of winter kill will ultimately kill the plant. We are trying to understand the cellular metabolism associated with the onset of rhizome dormancy and to connect the links between tiller/leaf senescence and rhizome metabolism using field grown plants from diverse populations, HTS and RNA-Seq.

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Genetic Resources for the Improvement of Switchgrass (Panicum virgatum L.) for Biomass and Forage

Switchgrass (Panicum virgatum L.) is an important forage and biomass species for many parts of the USA. Switchgrass can be of several ploidies. Octoploid cultivars are most often used in forage and conservation settings, while the tetraploid cultivars are mostly targeted for bioenergy end-uses, due to their higher biomass yields. Switchgrass populations also occur as upland and lowland ecotypes, and constitute different heterotic groups. Switchgrass is mostly an obligate outcrosser resulting in substantial genotypic and phenotypic variation within populations. In the last ~15 years, significant resources have been dedicated to both breeding and understanding the genomic makeup of this plant, with a focus on bioenergy. This investment has resulted in the development of elite lines as well as a considerable increase in available genetic, physiological, and biomass-related information. The United States Department of Agriculture-Agricultural Research Service has been a major player in these developments (Mitchell and Schmer, 2012; Vogel et al., 2011).

With significant improvements in DNA-sequencing technologies (High Throughput Sequencing, HTS), it has become possible to undertake large-scale analysis of both the genomic and functional genomic components of switchgrass. One such undertaking by the United States Department of Energy-Joint Genomics Institute has provided a draft assembly and annotation of the switchgrass genome (www.phytozome.org). This remarkable resource has permitted a complete utilization of HTS to analyze gene expression using RNA-Seq and related bioinformatic pipelines. Large-scale studies that are performed using field-grown plants and populations with well-characterized phenotypic traits, it increases the likelihood of discovering molecular events that underpin phenomena of interest. Even though lowland tetraploid cultivars have higher biomass yields than upland tetraploid cultivars, they can suffer significant winter-kill in more northern locations (Central Great Plains of the USA). Winter-kill is associated with the loss of rhizomes and other perenniating structures resulting in a complete or partial loss of tillering ability in the following seasons. Partial attrition of tiller production serves to limit new rhizome growth in successive years. One or more cycles of winter kill will ultimately kill the plant. We are trying to understand the cellular metabolism associated with the onset of rhizome dormancy and to connect the links between tiller/leaf senescence and rhizome metabolism using field grown plants from diverse populations, HTS and RNA-Seq.