Satellite Symposium 5: Molecular Breeding
Description
Hygromycin selection for the hpt gene, expressed from the CaMV-35S promoter, has been successful in transgenesis of a limited number of grass species. As an alternative to hpt selection Altpeter et al., (2000) reported successful transformation using paromomycin selection for the nptII gene expressed by the maize ubiquitin promoter. We have tested the utility of a number of selection cassettes using previously sporadically transformable species which nevertheless had very good tissue culture and regeneration protocols.
Citation
Dalton, S. J.; Robson, P.; Buanafina, M.; Bettany, A. J. E.; Timms, E.; and Morris, P., "A Comparison of Hygromycin and Paromomycin Selection Strategies in the Genetic Transformation of Seven Lolium, Festuca, Poa, and Agrostis Species" (2023). IGC Proceedings (1993-2023). 98.
https://uknowledge.uky.edu/igc/20/satellitesymposium5/98
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
A Comparison of Hygromycin and Paromomycin Selection Strategies in the Genetic Transformation of Seven Lolium, Festuca, Poa, and Agrostis Species
Hygromycin selection for the hpt gene, expressed from the CaMV-35S promoter, has been successful in transgenesis of a limited number of grass species. As an alternative to hpt selection Altpeter et al., (2000) reported successful transformation using paromomycin selection for the nptII gene expressed by the maize ubiquitin promoter. We have tested the utility of a number of selection cassettes using previously sporadically transformable species which nevertheless had very good tissue culture and regeneration protocols.
