Satellite Symposium 5: Molecular Breeding
Description
Using cell suspensions to transform some grass species by particle bombardment has a number of disadvantages including increased somoclonal variation in liquid cell culture and poor performance due to polysaccharide production. The use of calli avoids these problems, but the manipulation of calli through numerous media changes is laborious and time-consuming. We investigated a possible mechanism to facilitate the use of calli in transformation by immobilising calli on mesh.
Citation
Dalton, S. J.; Robson, P.; Buanafina, M.; Bettany, A. J. E.; Timms, E.; Wiffen, D.; and Morris, P., "Nylon Mesh as an Improved Support for Bombarded Calli or Cell Suspensions" (2023). IGC Proceedings (1993-2023). 97.
https://uknowledge.uky.edu/igc/20/satellitesymposium5/97
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Nylon Mesh as an Improved Support for Bombarded Calli or Cell Suspensions
Using cell suspensions to transform some grass species by particle bombardment has a number of disadvantages including increased somoclonal variation in liquid cell culture and poor performance due to polysaccharide production. The use of calli avoids these problems, but the manipulation of calli through numerous media changes is laborious and time-consuming. We investigated a possible mechanism to facilitate the use of calli in transformation by immobilising calli on mesh.
