Satellite Symposium 5: Molecular Breeding
Description
Key points:
- Molecular markers that allow the transfer of map information from one species to another are vital in comparative genetics.
- To identify potential anchor marker sequences more efficiently, we have established a bioinformatic pipeline that combines multi-species EST- and genome- sequence data.
- Taking advantage of information from a few related species, comparative EST sequence analysis identifies evolutionary conserved sequences in less well-characterised species in the same family.
- Alignment of evolutionary conserved EST sequences with corresponding genomic sequences defines sets of PCR primer sites flanking introns.
- Markers identified by this procedure will be readily transferable to other species since they are selected on the basis of their common evolutionary origin.
- We exemplify our procedure on legumes and grasses, where model plant studies and the genome- and EST-sequence data available have a potential impact on breeding crop species.
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
A Computational Pipeline for the Development of Comparative Anchor Tagged Sequence (CATS) Markers
Key points:
- Molecular markers that allow the transfer of map information from one species to another are vital in comparative genetics.
- To identify potential anchor marker sequences more efficiently, we have established a bioinformatic pipeline that combines multi-species EST- and genome- sequence data.
- Taking advantage of information from a few related species, comparative EST sequence analysis identifies evolutionary conserved sequences in less well-characterised species in the same family.
- Alignment of evolutionary conserved EST sequences with corresponding genomic sequences defines sets of PCR primer sites flanking introns.
- Markers identified by this procedure will be readily transferable to other species since they are selected on the basis of their common evolutionary origin.
- We exemplify our procedure on legumes and grasses, where model plant studies and the genome- and EST-sequence data available have a potential impact on breeding crop species.
