Satellite Symposium 5: Molecular Breeding

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Publication Date

2005

Location

Dublin Ireland

Description

Key points

  1. Molecular markers are effective tools to support traditional approaches in plant genetic resources management.
  2. Genetic diversity assessed for perennial ryegrass by AFLP analysis revealed differentiation of populations occurring in traditional Dutch grasslands from commercial varieties, but not from populations occurring in Dutch nature reserves.
  3. No specific conservation measures were recommended to maintain the genetic diversity of perennial ryegrass occurring in traditional Dutch grasslands.
  4. Pollination rates estimated by microsatellite analysis for a rejuvenated population of a perennial ryegrass genebank accession were very well described by an inverse quadratic function of inter-plant distance between potential mating pairs, while recorded flowering characteristics contributed only to a minor extent.
  5. Compared to variation in pollination rates, the genetic integrity of the rejuvenated perennial ryegrass accession was found to be more threatened by contamination, which indicated the need for improved regeneration protocols to prevent gene flow from other germplasm.

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Application of Molecular Markers in Genetic Resources Management of Perennial Ryegrass

Dublin Ireland

Key points

  1. Molecular markers are effective tools to support traditional approaches in plant genetic resources management.
  2. Genetic diversity assessed for perennial ryegrass by AFLP analysis revealed differentiation of populations occurring in traditional Dutch grasslands from commercial varieties, but not from populations occurring in Dutch nature reserves.
  3. No specific conservation measures were recommended to maintain the genetic diversity of perennial ryegrass occurring in traditional Dutch grasslands.
  4. Pollination rates estimated by microsatellite analysis for a rejuvenated population of a perennial ryegrass genebank accession were very well described by an inverse quadratic function of inter-plant distance between potential mating pairs, while recorded flowering characteristics contributed only to a minor extent.
  5. Compared to variation in pollination rates, the genetic integrity of the rejuvenated perennial ryegrass accession was found to be more threatened by contamination, which indicated the need for improved regeneration protocols to prevent gene flow from other germplasm.