Satellite Symposium 5: Molecular Breeding

Description

Key points:

  1. Molecular markers that allow the transfer of map information from one species to another are vital in comparative genetics.
  2. To identify potential anchor marker sequences more efficiently, we have established a bioinformatic pipeline that combines multi-species EST- and genome- sequence data.
  3. Taking advantage of information from a few related species, comparative EST sequence analysis identifies evolutionary conserved sequences in less well-characterised species in the same family.
  4. Alignment of evolutionary conserved EST sequences with corresponding genomic sequences defines sets of PCR primer sites flanking introns.
  5. Markers identified by this procedure will be readily transferable to other species since they are selected on the basis of their common evolutionary origin.
  6. We exemplify our procedure on legumes and grasses, where model plant studies and the genome- and EST-sequence data available have a potential impact on breeding crop species.

Share

COinS
 

A Computational Pipeline for the Development of Comparative Anchor Tagged Sequence (CATS) Markers

Key points:

  1. Molecular markers that allow the transfer of map information from one species to another are vital in comparative genetics.
  2. To identify potential anchor marker sequences more efficiently, we have established a bioinformatic pipeline that combines multi-species EST- and genome- sequence data.
  3. Taking advantage of information from a few related species, comparative EST sequence analysis identifies evolutionary conserved sequences in less well-characterised species in the same family.
  4. Alignment of evolutionary conserved EST sequences with corresponding genomic sequences defines sets of PCR primer sites flanking introns.
  5. Markers identified by this procedure will be readily transferable to other species since they are selected on the basis of their common evolutionary origin.
  6. We exemplify our procedure on legumes and grasses, where model plant studies and the genome- and EST-sequence data available have a potential impact on breeding crop species.