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Publication Date

1997

Location

Manitoba and Saskatchewan

Description

Cultivar development in alfalfa relies on heterosis, that has been mainly exploited by intercrossing selected clones from diverse gemplasm sources; an alternative way was proposed, based on developing double cross cultivars obtained by crossing clones selected for self-sterility, propagated by somatic seed. A different breeding strategy was recently emphasized based on population improvement through inbreeding and recurrent selection; in this case, self-sterility would be selected against. If self-sterility is determined by genetic load, the second strategy should be preferred, and selecting for self-fertility may bring about positive correlated responses for forage yield. Divergent selection for self-fertility was applied in a central Italian alfalfa landrace to test this hypothesis. Ten self-fertile and ten self-sterile plants were selected and hand-crossed without emasculation in a n(n-1) diallel; seeds of reciprocal crosses were pooled, obtaining 45 full-sib families per fertility group. Ten plants per family were evaluated for self-fertility. Selection was effective for self-fertility (h2 R=0.52), but not for self-sterility, a result expected if self-sterility is determined by genetic load. The forage yield of self-fertile progenies was 135% of the unselected control and 123% of the self-sterile progenies in a dense stand trial (first cut, seedling year), indicating that selection for self-fertility could be a tool for population improvement.

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Selection for Self-Fertility and Self-Fertility in Alfalfa as a Tool for Breeding Strategy Assessment

Manitoba and Saskatchewan

Cultivar development in alfalfa relies on heterosis, that has been mainly exploited by intercrossing selected clones from diverse gemplasm sources; an alternative way was proposed, based on developing double cross cultivars obtained by crossing clones selected for self-sterility, propagated by somatic seed. A different breeding strategy was recently emphasized based on population improvement through inbreeding and recurrent selection; in this case, self-sterility would be selected against. If self-sterility is determined by genetic load, the second strategy should be preferred, and selecting for self-fertility may bring about positive correlated responses for forage yield. Divergent selection for self-fertility was applied in a central Italian alfalfa landrace to test this hypothesis. Ten self-fertile and ten self-sterile plants were selected and hand-crossed without emasculation in a n(n-1) diallel; seeds of reciprocal crosses were pooled, obtaining 45 full-sib families per fertility group. Ten plants per family were evaluated for self-fertility. Selection was effective for self-fertility (h2 R=0.52), but not for self-sterility, a result expected if self-sterility is determined by genetic load. The forage yield of self-fertile progenies was 135% of the unselected control and 123% of the self-sterile progenies in a dense stand trial (first cut, seedling year), indicating that selection for self-fertility could be a tool for population improvement.