Description
Metabolically inactivated tall fescue (Festuca arundinacea Schreb.) protoplasts and X-ray irradiated Italian ryegrass (Lolium multiflorum Lam.) protoplasts were electrofused resulting in the regeneration of twenty-six plantlets. Southern blot analysis using three mitochondrial probes and random amplified polymorphic DNA (RAPD) analysis revealed that the regenerants were intergeneric somatic hybrids. All regenerated plants were male sterile and some of them had stamens that exhibited the pistillode appearance.
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Characterization of Somatic Hybrids Between Festuca Arundinacea and Lolium Multiflorum
Metabolically inactivated tall fescue (Festuca arundinacea Schreb.) protoplasts and X-ray irradiated Italian ryegrass (Lolium multiflorum Lam.) protoplasts were electrofused resulting in the regeneration of twenty-six plantlets. Southern blot analysis using three mitochondrial probes and random amplified polymorphic DNA (RAPD) analysis revealed that the regenerants were intergeneric somatic hybrids. All regenerated plants were male sterile and some of them had stamens that exhibited the pistillode appearance.
