Publication Date

1993

Description

Three growing methods (field sward, field spaced plants, glasshouse plants) were compared for the morphological assessment of white clover (Trifolium repens L.) cultivar purity. Three cerlified (breeders, basic and first generation) seed-lots of cv. Grasslands Kopu and one first generation Kopu seed lot which had been mixed with seed of another cultivnr (10:1) were used to generate data. After analysis of variance (ANOVA), significant (P<0.05) reductions from the breeders seed lot siandnrd for leaf size, stolon number and leaf length/width ratio were recorded for the mixed seed· 101 in nil three growing methods, and for petiole length with the sward method only. With the glasshouse and spaced-plant melhods, slolon numbers of the basic and first generation seed-lots were reduced (P<0.05), while leaf size was lower (P<0.05) for the first generation seed-lot in lhe glasshouse. However, under OECD certification requirements, leaf size of the mixed seed lot was the only characler for which "off types" were detecled. Data coefficients of variation were no greater for the glasshouse method than the required OECD plot-test method.

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Growing Methods for the Morphological Assessment of White Clover Cultivars Purity

Three growing methods (field sward, field spaced plants, glasshouse plants) were compared for the morphological assessment of white clover (Trifolium repens L.) cultivar purity. Three cerlified (breeders, basic and first generation) seed-lots of cv. Grasslands Kopu and one first generation Kopu seed lot which had been mixed with seed of another cultivnr (10:1) were used to generate data. After analysis of variance (ANOVA), significant (P<0.05) reductions from the breeders seed lot siandnrd for leaf size, stolon number and leaf length/width ratio were recorded for the mixed seed· 101 in nil three growing methods, and for petiole length with the sward method only. With the glasshouse and spaced-plant melhods, slolon numbers of the basic and first generation seed-lots were reduced (P<0.05), while leaf size was lower (P<0.05) for the first generation seed-lot in lhe glasshouse. However, under OECD certification requirements, leaf size of the mixed seed lot was the only characler for which "off types" were detecled. Data coefficients of variation were no greater for the glasshouse method than the required OECD plot-test method.