Publication Date
1993
Description
Escaped plants of white clover which could not be identified by variety were collected and were grown in pots filled with a perlite contained nutrient solution in a growlh chamber. On 16 of the collecled plants, the change of the nitrogenase activity by treatments with or without nitrogen (20 ppm N, NaNO3) was measured by gas chromatogrnphy and the variation of peroxidase isozyme was analysed by means of slab acrylamide gel electrophoresis. The studied plants of white clover (Trifolium repens L.) were classified into 5 types by the response pattern of nitrogenase to N applied. Six bands showing peroxidase activity were detected in the extract from the leaflets and the plants were classified into 4 types by the banding patterns of peroxidase isozyme. The specific relationship between banding type of peroxidase isozyme and the inhibition of nitrogenase by N applied was suggested in white clover ecotypes.
Citation
Sugawara, K; Nishiwaki, A; and Yashima, Y, "Effect of Nitrogen Application on Symbiotic Nitrogen Fixation of White Clover Ecotypes Collected in North-Eastern Japan" (2024). IGC Proceedings (1993-2023). 4.
https://uknowledge.uky.edu/igc/1993/session34/4
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Effect of Nitrogen Application on Symbiotic Nitrogen Fixation of White Clover Ecotypes Collected in North-Eastern Japan
Escaped plants of white clover which could not be identified by variety were collected and were grown in pots filled with a perlite contained nutrient solution in a growlh chamber. On 16 of the collecled plants, the change of the nitrogenase activity by treatments with or without nitrogen (20 ppm N, NaNO3) was measured by gas chromatogrnphy and the variation of peroxidase isozyme was analysed by means of slab acrylamide gel electrophoresis. The studied plants of white clover (Trifolium repens L.) were classified into 5 types by the response pattern of nitrogenase to N applied. Six bands showing peroxidase activity were detected in the extract from the leaflets and the plants were classified into 4 types by the banding patterns of peroxidase isozyme. The specific relationship between banding type of peroxidase isozyme and the inhibition of nitrogenase by N applied was suggested in white clover ecotypes.
