Publication Date
1993
Description
Azide-resistant mutants of Rhizobium legumnosarum bv, trifolii strain NZPS61 were isolated. Mutations conferring azide resistance appeared at a frequency of 1.6x t0·7 on minimal media containing 25 µglml sodium azide. Two classes of mutants were observed with respect to the nitrogen source in the growth media. Mutants in the first class showed azide resistance in the presence of both nitrate and ammonia whereas the mutants in the second class showed azide resistance only in the presence of nitrate. Eleven azide-resistant mutants were characterised for their symbiotic ability with white clover. All the mutants were similar to their parent strain NZPS61 with respect to nitrogen fixatiori efficiency.
Citation
Sharma, Shashi Bhushan; Sakadevan, K; and Corner, R, "Symbiotic Characterization of Azide-Resistant Mutants of Rhizobium leguminosarum by trifolii" (2024). IGC Proceedings (1993-2023). 3.
https://uknowledge.uky.edu/igc/1993/session34/3
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Symbiotic Characterization of Azide-Resistant Mutants of Rhizobium leguminosarum by trifolii
Azide-resistant mutants of Rhizobium legumnosarum bv, trifolii strain NZPS61 were isolated. Mutations conferring azide resistance appeared at a frequency of 1.6x t0·7 on minimal media containing 25 µglml sodium azide. Two classes of mutants were observed with respect to the nitrogen source in the growth media. Mutants in the first class showed azide resistance in the presence of both nitrate and ammonia whereas the mutants in the second class showed azide resistance only in the presence of nitrate. Eleven azide-resistant mutants were characterised for their symbiotic ability with white clover. All the mutants were similar to their parent strain NZPS61 with respect to nitrogen fixatiori efficiency.