US Patent Number
4,473,640
Publication Date
1984
Abstract
The invention relates to a method for hydrolyzing drug glucuronic acid conjugates present in mammalian body fluids, the conjugates being derived from a narcotic analgesic, antagonist, or agonist-antagonist whose metabolism includes conjugation with glucuronic acid. The method comprises incubating the body fluid sample at from about 60 to about 70° C., for at least about 1 hour, with 3-glucuronidase derived from Patella vulgata, and substantially increases the sensitivity of chromatographic techniques for the detection of morphine and its analogues.
Application Number
384,719
Filing Date
Jun. 3, 1982
Recommended Citation
Combie, Joan; Blake, Jerry W.; Nugent, Thomas E.; and Tobin, Thomas, "DETECTION OF MORPHINE AND ITS ANALOGUES USING ENZYMATIC HYDROLYSIS" (1984). Veterinary Science Faculty Patents. 18.
https://uknowledge.uky.edu/gluck_patents/18
