Genome-Wide Analysis of ATP-Binding Cassette (ABC) Transporters in the Sweetpotato Whitefly, Bemisia tabaci

Authors

Lixia Tian, Chinese Academy of Agricultural Sciences, China
Tianxue Song, Northeast Agricultural University, China
Rongjun He, Chinese Academy of Agricultural Sciences, China
Yang Zeng, Chinese Academy of Agricultural Sciences, China
Wen Xie, Chinese Academy of Agricultural Sciences, China
Qingjun Wu, Chinese Academy of Agricultural Sciences, China
Shaoli Wang, Chinese Academy of Agricultural Sciences, China
Xuguo Zhou, University of KentuckyFollow
Youjun Zhang, Chinese Academy of Agricultural Sciences, China

Abstract

Background: ABC transporter superfamily is one of the largest and ubiquitous groups of proteins. Because of their role in detoxification, insect ABC transporters have gained more attention in recent years. In this study, we annotated ABC transporters from a newly sequenced sweetpotato whitefly genome. Bemisia tabaci Q biotype is an emerging global invasive species that has caused extensive damages to field crops as well as ornamental plants.

Results: A total of 55 ABC transporters containing all eight described subfamilies (A to H) were identified in the B. tabaci Q genome, including 8 ABCAs, 3 ABCBs, 6 ABCCs, 2 ABCDs, 1 ABCE, 3 ABCFs, 23 ABCGs and 9 ABCHs. In comparison to other species, subfamilies G and H in both phloem- and blood-sucking arthropods are expanded. The temporal expression profiles of these 55 ABC transporters throughout B. tabaci developmental stages and their responses to imidacloprid, a neonicotinoid insecticide, were investigated using RNA-seq analysis. Furthermore, the mRNA expression of 24 ABC transporters (44% of the total) representing all eight subfamilies was confirmed by the quantitative real-time PCR (RT-qPCR). Furthermore, mRNA expression levels estimated by RT-qPCR and RNA-seq analyses were significantly correlated (r = 0.684, p < 0.01).

Conclusions: It is the first genome-wide analysis of the entire repertoire of ABC transporters in B. tabaci. The identification of these ABC transporters, their temporal expression profiles during B. tabaci development, and their response to a neonicotinoid insecticide lay the foundation for functional genomic understanding of their contribution to the invasiveness of B. tabaci.

Document Type

Article

Publication Date

4-26-2017

Notes/Citation Information

Published in BMC Genomics, v. 18, 330, p. 1-16.

© The Author(s). 2017

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Digital Object Identifier (DOI)

https://doi.org/10.1186/s12864-017-3706-6

Funding Information

This work was supported by the National Natural Science Foundation of China (31420103919 and 31672032), and China Agriculture Research System (CARS-26 10).

Related Content

Bemisia tabaci Q ABC transporter dataset will be available from the corresponding authors on reasonable requests. Transcriptome datasets of B. tabaci Q at different development stages and following the exposure to imidacloprid insecticide have been deposited, respectively, in the Sequence Read Archive (SRA) database under the accession number SRP064690 and SRP090405.

Repository Citation

Tian, Lixia; Song, Tianxue; He, Rongjun; Zeng, Yang; Xie, Wen; Wu, Qingjun; Wang, Shaoli; Zhou, Xuguo; and Zhang, Youjun, "Genome-Wide Analysis of ATP-Binding Cassette (ABC) Transporters in the Sweetpotato Whitefly, Bemisia tabaci" (2017). Entomology Faculty Publications. 130.
https://uknowledge.uky.edu/entomology_facpub/130