Publication Date
1997
Description
In the present study seed proteins and isozymes of varieties and lines of Festuca pratensis and Festulolium were investigated with the aim of finding methods for the discrimination of meadow fescue varieties and the identification of Festulolium hybrids. The banding patterns of ultrathin-layer isoelectric focusing (UTLIEF) of bulked seed samples allowed a clear distinction of Festulolium hybrids of different crossing combinations from each other and in most cases also from their parental species. Despite the outcrossing character, identification of some hybrids and crossing parents was also achieved using protein patterns of single seeds. By means of isozyme electrophoresis of Festuca pratensis varieties the genotype and allele frequencies of 5 loci were detected. The 10 analysed varieties were successfully differentiated by comparisons of allele frequencies or genotype frequencies using Likelihood ratio test and c2-test, respectively. Both methods, UTLIEF and isozyme electrophoresis, proved to be suitable for reliable distinguishing of Festuca pratensis and Festulolium varieties and lines.
Citation
Hahn, H; Schoberlein, W; and Forster, K, "Differentiation of Festuca pratensis Varieties and Festulolium Hybrids by Electrophoresis" (2024). IGC Proceedings (1997-2023). 11.
https://uknowledge.uky.edu/igc/1997/session25/11
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Differentiation of Festuca pratensis Varieties and Festulolium Hybrids by Electrophoresis
In the present study seed proteins and isozymes of varieties and lines of Festuca pratensis and Festulolium were investigated with the aim of finding methods for the discrimination of meadow fescue varieties and the identification of Festulolium hybrids. The banding patterns of ultrathin-layer isoelectric focusing (UTLIEF) of bulked seed samples allowed a clear distinction of Festulolium hybrids of different crossing combinations from each other and in most cases also from their parental species. Despite the outcrossing character, identification of some hybrids and crossing parents was also achieved using protein patterns of single seeds. By means of isozyme electrophoresis of Festuca pratensis varieties the genotype and allele frequencies of 5 loci were detected. The 10 analysed varieties were successfully differentiated by comparisons of allele frequencies or genotype frequencies using Likelihood ratio test and c2-test, respectively. Both methods, UTLIEF and isozyme electrophoresis, proved to be suitable for reliable distinguishing of Festuca pratensis and Festulolium varieties and lines.
