Abstract
Macrophages, one of the most important phagocytic cells of the immune system, are highly plastic and are known to exhibit diverse roles under different pathological conditions. The ability to repolarize macrophages from pro-inflammatory (M1) to anti-inflammatory (M2) or vice versa offers a promising therapeutic approach for treating various diseases such as traumatic injury and cancer. Herein, it is demonstrated that macrophage-engineered vesicles (MEVs) generated by disruption of macrophage cellular membranes can be used as nanocarriers capable of reprogramming macrophages and microglia toward either pro- or anti-inflammatory phenotypes. MEVs can be produced at high yields and easily loaded with diagnostic molecules or chemotherapeutics and delivered to both macrophages and cancer cells in vitro and in vivo. Overall, MEVs show promise as potential delivery vehicles for both therapeutics and their ability to controllably modulate macrophage/microglia inflammatory phenotypes.
Document Type
Article
Publication Date
1-26-2021
Digital Object Identifier (DOI)
https://doi.org/10.1021/acsomega.0c05632
Funding Information
Support for this work was provided by the Kentucky Pediatric Cancer Research Trust Fund (PON2 728 2000002500).
Related Content
The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acsomega.0c05632. It is also available for download as the additional file listed at the end of this record.
Repository Citation
Neupane, Khaga R.; McCorkle, J. Robert; Kopper, Timothy J.; Lakes, Jourdan E.; Aryal, Surya P.; Abdullah, Masud; Snell, Aaron A.; Gensel, John C.; Kolesar, Jill M.; and Richards, Christopher I., "Macrophage-Engineered Vesicles for Therapeutic Delivery and Bidirectional Reprogramming of Immune Cell Polarization" (2021). Spinal Cord and Brain Injury Research Center Faculty Publications. 39.
https://uknowledge.uky.edu/scobirc_facpub/39
Vesicle delivery and cytokine production
Included in
Immunology and Infectious Disease Commons, Medical Physiology Commons, Neurology Commons
Notes/Citation Information
Published in ACS Omega, v. 6, issue 5.
© 2021 The Authors
This is an open access article published under a Creative Commons Non-Commercial No-Derivative Works (CC-BY-NC-ND) Attribution License, which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.