Two highly active trifunctional hemicellulases were constructed by linking the catalytic portion of a xylanase with an arabinofuranosidase and a xylosidase, using either flexible peptide linkers or linkers containing a cellulose-binding domain. The multifunctional enzymes retain the parental enzyme properties and exhibit synergistic effects in hydrolysis of natural xylans and corn stover.
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This work was supported by a grant from the U.S. Department of Agriculture to L.Y. (2006-35504-17413).
Supplemental material for this article may be found at http://aem.asm.org/.
Fan, Zhanmin; Wagschal, Kurt; Chen, Wei; Montross, Michael D.; Lee, Charles C.; and Yuan, Ling, "Multimeric Hemicellulases Facilitate Biomass Conversion" (2009). Plant and Soil Sciences Faculty Publications. 91.
Supplemental Information: Data on PCR primers used in construction of the multimeric enzymes (Table S1) and construction of the multimeric enzymes (AXX and CBD-AXX).