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Abstract
Two highly active trifunctional hemicellulases were constructed by linking the catalytic portion of a xylanase with an arabinofuranosidase and a xylosidase, using either flexible peptide linkers or linkers containing a cellulose-binding domain. The multifunctional enzymes retain the parental enzyme properties and exhibit synergistic effects in hydrolysis of natural xylans and corn stover.
Document Type
Article
Publication Date
3-2009
Digital Object Identifier (DOI)
https://doi.org/10.1128/AEM.02181-08
Funding Information
This work was supported by a grant from the U.S. Department of Agriculture to L.Y. (2006-35504-17413).
Related Content
Supplemental material for this article may be found at http://aem.asm.org/.
Repository Citation
Fan, Zhanmin; Wagschal, Kurt; Chen, Wei; Montross, Michael D.; Lee, Charles C.; and Yuan, Ling, "Multimeric Hemicellulases Facilitate Biomass Conversion" (2009). Plant and Soil Sciences Faculty Publications. 91.
https://uknowledge.uky.edu/pss_facpub/91
Supplemental Information: Data on PCR primers used in construction of the multimeric enzymes (Table S1) and construction of the multimeric enzymes (AXX and CBD-AXX).
Included in
Environmental Microbiology and Microbial Ecology Commons, Enzymes and Coenzymes Commons, Plant Sciences Commons, Soil Science Commons
Notes/Citation Information
Published in Applied and Environmental Microbiology, v. 75, no. 6, p. 1754-1757.
Copyright © 2009, American Society for Microbiology. All Rights Reserved.
The copyright holder has granted the permission for posting the article here.