Abstract
In the female gamete of flowering plants, sperm nuclear migration is controlled by a constant inward movement of actin filaments (F-actin) for successful fertilization. This dynamic F-actin movement is ARP2/3-independent, raising the question of how actin nucleation and polymerization is controlled in the female gamete. Using confocal microscopy live-cell imaging in combination with a pharmacological approach, we assessed the involvement of another group of actin nucleators, formins, in F-actin inward movement in the central cell of Arabidopsis thaliana. We identify that the inhibition of the formin function, by formin inhibitor SMIFH2, significantly reduced the dynamic inward movement of F-actin in the central cell, indicating that formins play a major role in actin nucleation required for F-actin inward movement in the central cell.
Document Type
Article
Publication Date
5-4-2021
Digital Object Identifier (DOI)
https://doi.org/10.1080/15592324.2021.1920192
Funding Information
This work was supported by the National Science Foundation [IOS-1928836]; National Institute of Food and Agriculture [1014280].
Repository Citation
Ali, Mohammad F. and Kawashima, Tomokazu, "Formins Control Dynamics of F-Actin in the Central Cell of Arabidopsis thaliana" (2021). Plant and Soil Sciences Faculty Publications. 157.
https://uknowledge.uky.edu/pss_facpub/157
Notes/Citation Information
Published in Plant Signaling & Behavior, v. 16, issue 8.
This is an Accepted Manuscript version of the following article, accepted for publication in Plant Signaling & Behavior. Ali, M. F., & Kawashima, T. (2021). Formins control dynamics of F-actin in the central cell of Arabidopsis thaliana. Plant Signaling & Behavior, 16(8), 1920192. https://doi.org/10.1080/15592324.2021.1920192
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