Archived
This content is available here strictly for research, reference, and/or recordkeeping and as such it may not be fully accessible. If you work or study at University of Kentucky and would like to request an accessible version, please use the SensusAccess Document Converter.
Abstract
In the female gamete of flowering plants, sperm nuclear migration is controlled by a constant inward movement of actin filaments (F-actin) for successful fertilization. This dynamic F-actin movement is ARP2/3-independent, raising the question of how actin nucleation and polymerization is controlled in the female gamete. Using confocal microscopy live-cell imaging in combination with a pharmacological approach, we assessed the involvement of another group of actin nucleators, formins, in F-actin inward movement in the central cell of Arabidopsis thaliana. We identify that the inhibition of the formin function, by formin inhibitor SMIFH2, significantly reduced the dynamic inward movement of F-actin in the central cell, indicating that formins play a major role in actin nucleation required for F-actin inward movement in the central cell.
Document Type
Article
Publication Date
5-4-2021
Digital Object Identifier (DOI)
https://doi.org/10.1080/15592324.2021.1920192
Funding Information
This work was supported by the National Science Foundation [IOS-1928836]; National Institute of Food and Agriculture [1014280].
Repository Citation
Ali, Mohammad F. and Kawashima, Tomokazu, "Formins Control Dynamics of F-Actin in the Central Cell of Arabidopsis thaliana" (2021). Plant and Soil Sciences Faculty Publications. 157.
https://uknowledge.uky.edu/pss_facpub/157
Notes/Citation Information
Published in Plant Signaling & Behavior, v. 16, issue 8.
This is an Accepted Manuscript version of the following article, accepted for publication in Plant Signaling & Behavior. Ali, M. F., & Kawashima, T. (2021). Formins control dynamics of F-actin in the central cell of Arabidopsis thaliana. Plant Signaling & Behavior, 16(8), 1920192. https://doi.org/10.1080/15592324.2021.1920192
It is deposited under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.