COP1, a Negative Regulator of Photomorphogenesis, Positively Regulates Plant Disease Resistance via Double-Stranded RNA Binding Proteins

Authors

Gah-Hyun Lim, University of KentuckyFollow
Timothy Hoey, University of KentuckyFollow
Shifeng Zhu, University of KentuckyFollow
Marion Clavel, Université de Perpignan Via Domitia, France
Keshun Yu, University of KentuckyFollow
Duroy Navarre, US Department of Agriculture
Aardra Kachroo, University of KentuckyFollow
Jean-Marc Deragon, Université de Perpignan Via Domitia, France
Pradeep Kachroo, University of KentuckyFollow

Abstract

The E3 ubiquitin ligase COP1 (Constitutive Photomorphogenesis 1) is a well known component of the light-mediated plant development that acts as a repressor of photomorphogenesis. Here we show that COP1 positively regulates defense against turnip crinkle virus (TCV) and avrRPM1 bacteria by contributing to stability of resistance (R) protein HRT and RPM1, respectively. HRT and RPM1 levels and thereby pathogen resistance is significantly reduced in the cop1 mutant background. Notably, the levels of at least two double-stranded RNA binding (DRB) proteins DRB1 and DRB4 are reduced in the cop1 mutant background suggesting that COP1 affects HRT stability via its effect on the DRB proteins. Indeed, a mutation in either drb1 or drb4 resulted in degradation of HRT. In contrast to COP1, a multi-subunit E3 ligase encoded by anaphase-promoting complex (APC) 10 negatively regulates DRB4 and TCV resistance but had no effect on DRB1 levels. We propose that COP1-mediated positive regulation of HRT is dependent on a balance between COP1 and negative regulators that target DRB1 and DRB4.

Document Type

Article

Publication Date

3-7-2018

Notes/Citation Information

Published in PLOS Pathogens, v. 14, no, 3, e1006894, p. 1-23.

This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

Digital Object Identifier (DOI)

https://doi.org/10.1371/journal.ppat.1006894

Funding Information

This work was supported by grants 0641576 and 0749731 from the National Science Foundation to PK. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Related Content

All relevant data are within the paper and its Supporting Information files.

Repository Citation

Lim, Gah-Hyun; Hoey, Timothy; Zhu, Shifeng; Clavel, Marion; Yu, Keshun; Navarre, Duroy; Kachroo, Aardra; Deragon, Jean-Marc; and Kachroo, Pradeep, "COP1, a Negative Regulator of Photomorphogenesis, Positively Regulates Plant Disease Resistance via Double-Stranded RNA Binding Proteins" (2018). Plant Pathology Faculty Publications. 75.
https://uknowledge.uky.edu/plantpath_facpub/75