A Feedback Regulatory Loop between G3P and Lipid Transfer Proteins DIR1 and AZI1 Mediates Azelaic-Acid-Induced Systemic Immunity

Authors

Keshun Yu, University of KentuckyFollow
Juliana Moreira Soares, University of KentuckyFollow
Mihir Kumar Mandal, University of KentuckyFollow
Caixia Wang, University of KentuckyFollow
Bidisha Chanda, University of KentuckyFollow
Andrew N. Gifford, Brookhaven National Laboratory
Joanna S. Fowler, Brookhaven National Laboratory
Duroy Navarre, Washington State University
Aardra Kachroo, University of KentuckyFollow
Pradeep Kachroo, University of KentuckyFollow

Abstract

Systemic acquired resistance (SAR), a highly desirable form of plant defense, provides broad-spectrum immunity against diverse pathogens. The recent identification of seemingly unrelated chemical inducers of SAR warrants an investigation of their mutual interrelationships. We show that SAR induced by the dicarboxylic acid azelaic acid (AA) requires the phosphorylated sugar derivative glycerol-3-phosphate (G3P). Pathogen inoculation induced the release of free unsaturated fatty acids (FAs) and thereby triggered AA accumulation, because these FAs serve as precursors for AA. AA accumulation in turn increased the levels of G3P, which is required for AA-conferred SAR. The lipid transfer proteins DIR1 and AZI1, both of which are required for G3P- and AA-induced SAR, were essential for G3P accumulation. Conversely, reduced G3P resulted in decreased AZI1 and DIR1 transcription. Our results demonstrate that an intricate feedback regulatory loop among G3P, DIR1, and AZI1 regulates SAR and that AA functions upstream of G3P in this pathway.

Document Type

Article

Publication Date

4-25-2013

Notes/Citation Information

Published in Cell Reports, v. 3, no. 4, p. 1266-1278.

Copyright © 2013 The Authors. Published by Elsevier Inc. All rights reserved.

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

Digital Object Identifier (DOI)

http://dx.doi.org/10.1016/j.celrep.2013.03.030

Repository Citation

Yu, Keshun; Soares, Juliana Moreira; Mandal, Mihir Kumar; Wang, Caixia; Chanda, Bidisha; Gifford, Andrew N.; Fowler, Joanna S.; Navarre, Duroy; Kachroo, Aardra; and Kachroo, Pradeep, "A Feedback Regulatory Loop between G3P and Lipid Transfer Proteins DIR1 and AZI1 Mediates Azelaic-Acid-Induced Systemic Immunity" (2013). Plant Pathology Faculty Publications. 24.
https://uknowledge.uky.edu/plantpath_facpub/24