Smooth-Muscle BMAL1 Participates in Blood Pressure Circadian Rhythm Regulation

Authors

Zhongwen Xie, University of KentuckyFollow
Wen Su, University of KentuckyFollow
Shu Liu, University of KentuckyFollow
Guogang Zhao, University of KentuckyFollow
Karyn Esser, University of KentuckyFollow
Elizabeth A. Schroder, University of KentuckyFollow
Mellani Lefta, University of KentuckyFollow
Harald M. Stauss, University of Iowa
Zhenheng Guo, University of KentuckyFollow
Ming Cui Gong, University of KentuckyFollow

Abstract

As the central pacemaker, the suprachiasmatic nucleus (SCN) has long been considered the primary regulator of blood pressure circadian rhythm; however, this dogma has been challenged by the discovery that each of the clock genes present in the SCN is also expressed and functions in peripheral tissues. The involvement and contribution of these peripheral clock genes in the circadian rhythm of blood pressure remains uncertain. Here, we demonstrate that selective deletion of the circadian clock transcriptional activator aryl hydrocarbon receptor nuclear translocator-like (Bmal1) from smooth muscle, but not from cardiomyocytes, compromised blood pressure circadian rhythm and decreased blood pressure without affecting SCN-controlled locomotor activity in murine models. In mesenteric arteries, BMAL1 bound to the promoter of and activated the transcription of Rho-kinase 2 (Rock2), and Bmal1 deletion abolished the time-of-day variations in response to agonist-induced vasoconstriction, myosin phosphorylation, and ROCK2 activation. Together, these data indicate that peripheral inputs contribute to the daily control of vasoconstriction and blood pressure and suggest that clock gene expression outside of the SCN should be further evaluated to elucidate pathogenic mechanisms of diseases involving blood pressure circadian rhythm disruption.

Document Type

Article

Publication Date

1-2015

Notes/Citation Information

Published in The Journal of Clinical Investigation, v. 125, no. 1, p. 324-336.

© 2014, The American Society for Clinical Investigation.

The copyright holders have granted the permission for posting the article here.

Digital Object Identifier (DOI)

http://dx.doi.org/10.1172/JCI76881

Funding Information

This work was supported by NIH grants HL088389 (to Z. Guo), HL106843 (to M.C. Gong and Z. Guo), and RC1ES018636 (to K. Esser and M.C. Gong) as well as a National Institute of General Medical Sciences grant (P20 GM103527-05 to L. Cassis).

Repository Citation

Xie, Zhongwen; Su, Wen; Liu, Shu; Zhao, Guogang; Esser, Karyn; Schroder, Elizabeth A.; Lefta, Mellani; Stauss, Harald M.; Guo, Zhenheng; and Gong, Ming Cui, "Smooth-Muscle BMAL1 Participates in Blood Pressure Circadian Rhythm Regulation" (2015). Physiology Faculty Publications. 61.
https://uknowledge.uky.edu/physiology_facpub/61