Responses of skeletal muscle to mechanical stimuli in female rats following and during muscle disuse atrophy

Authors

• Alexander B. Sklivas, University of KentuckyFollow
• Zachary R. Hettinger, Oklahoma Medical Research Foundation
• Sarah Rose, University of Kentucky
• Alessandra Mantuano, University of Kentucky
• Amy L. Confides, University of KentuckyFollow
• Sandra Rigsby, Oklahoma Medical Research Foundation
• Frederick F. Peelor, Oklahoma Medical Research Foundation
• Benjamin F. Miller, Oklahoma Medical Research Foundation
• Timothy A. Butterfield, University of KentuckyFollow
• Esther E. Dupont-Versteegden, University of KentuckyFollow

Abstract

The purpose of this study was to investigate the ability of mechanotherapy to enhance recovery or prevent loss of muscle size with disuse in female rats. Female F344/BN rats were assigned to weight bearing (WB), hindlimb suspended (HS) for 14 days with reambulation for 7 days without mechanotherapy or reambulation (RA) with mechanotherapy (RAM) (study 1), or to WB, HS for 7 days, with HS mechanotherapy (HSM) or without mechanotherapy (study 2) to gastrocnemius muscle. Muscle fiber cross-sectional area (CSA) and fiber type, collagen, satellite cell number, and protein synthesis (k_syn) and degradation (k_deg) were assessed. Study 1: muscle weight, but not CSA, was higher in RAM compared with HS, but CSA was higher in RA compared with HS. Myofibrillar k_syn was higher in RA and RAM compared to WB and HS but not different between RA and RAM. Myofibrillar k_deg was lower with mechanotherapy compared to HS. Study 2: muscle weight, CSA, and myofibrillar k_syn and k_deg were not different with mechanotherapy. Collagen content was lower with mechanotherapy but collagen k_syn was not. Mechanotherapy was not associated with changes in fiber type, satellite cell, or myonuclear number in either study. Compared to males, female rats had less muscle loss with HS, which was associated with less loss of myofibrillar k_syn. Recovery from atrophy was associated with higher k_syn in female and lower k_deg in male rats. Female rat muscles do not exhibit a growth response to mechanotherapy with disuse or reambulation. Furthermore, male and female rats show distinct responses to different mechanical stimuli.

Document Type

Article

Publication Date

3-1-2025

Notes/Citation Information

Publisher Copyright: © 2025 American Physiological Society. All rights reserved.

Digital Object Identifier (DOI)

10.1152/japplphysiol.00802.2024

Archival?

Archival

Repository Citation

Sklivas, Alexander B.; Hettinger, Zachary R.; Rose, Sarah; Mantuano, Alessandra; Confides, Amy L.; Rigsby, Sandra; Peelor, Frederick F.; Miller, Benjamin F.; Butterfield, Timothy A.; and Dupont-Versteegden, Esther E., "Responses of skeletal muscle to mechanical stimuli in female rats following and during muscle disuse atrophy" (2025). Physiology Faculty Publications. 261.
https://uknowledge.uky.edu/physiology_facpub/261