Multidimensional biocircuitry of exercise adaptation

Authors

• Jeremy S. McAdam, Institute for Human & Machine Cognition
• Zachary A. Graham, Institute for Human & Machine Cognition
• S. Craig Tuggle, Institute for Human & Machine Cognition
• Kaleen M. Lavin, Institute for Human & Machine Cognition
• Marcas M. Bamman, Institute for Human & Machine Cognition
• Jeremy S. McAdam, Center for Exercise Medicine
• Zachary A. Graham, Center for Exercise Medicine
• Brandon Peoples, Center for Exercise Medicine
• Regina S. Seay, Center for Exercise Medicine
• Kaleen M. Lavin, Center for Exercise Medicine
• Amber B. Gargus, Center for Exercise Medicine
• Sufen Yang, Center for Exercise Medicine
• Samia M. O’Bryan, Center for Exercise Medicine
• Devin J. Drummer, Center for Exercise Medicine
• Christian J. Kelley, Center for Exercise Medicine
• Margaret B. Bell, Center for Exercise Medicine
• Inmaculada Aban, Center for Exercise Medicine
• Gary R. Cutter, Center for Exercise Medicine
• Marcas M. Bamman, Center for Exercise Medicine
• Michael P. Craig, Wright State University Boonshoft School of Medicine
• Jin Zhang, Wright State University Boonshoft School of Medicine
• Akshay Hira, Wright State University Boonshoft School of Medicine
• Timothy J. Broderick, Wright State University Boonshoft School of Medicine
• Madhavi P. Kadakia, Wright State University Boonshoft School of Medicine
• Zachary A. Graham, VA Medical Center
• Kalyani Peri, Department of Biostatistics
• Margaret B. Bell, Department of Biostatistics
• Inmaculada Aban, Department of Biostatistics

Abstract

In a randomized, dose-response trial, we used molecular and phenomic profiling to compare responses with traditional moder ate-intensity endurance and resistance training (TRAD) versus high-intensity tactical training (HITT) that encompassed explosive whole-body interval training and high-intensity resistance training. Ninety-four participants (18–27 yr) completed 12 wk of TRAD or HITT followed by 4 wk of detraining. Although similar performance and body composition improvements were observed in response to HITT and TRAD, some dose-dependent differences were observed for: 1) ex vivo muscle tissue changes in myofiber size, capillarization, satellite cell frequency, and mitochondrial function and 2) differential gene expression (DGE) of muscle and serum exosomal miRNAs (miRs). However, these dose-dependent ex vivo muscle adaptations were overshadowed by wide-rang ing interindividual response heterogeneity (IRH). We therefore explored response heterogeneity by first establishing minimum clinically important difference (MCID) scores to classify each participant based on MCIDs for functional muscle quality (fMQ) and cardiorespiratory fitness (CRF) and then modeling all data based on MCID classification. Using higher-order singular value decomposition (HOSVD), we established multidimensional biocircuitry linked to interindividual response heterogeneity that identi f ied the most influential features across lifestyle, body composition, performance, ex vivo muscle tissue, and miRNA mapping domains. Via cross-comparison of MCID-linked miRs identified via DGE and HOSVD, nine miRs emerged as robust features of training adaptability, providing new insights into the integrated biocircuitry driving IRH.

Document Type

Article

Publication Date

9-1-2025

Notes/Citation Information

Publisher Copyright: © 2025, American Physiological Society. All rights reserved.

Digital Object Identifier (DOI)

10.1152/physiolgenomics.00068.2025

Archival?

Archival

Repository Citation

McAdam, Jeremy S.; Graham, Zachary A.; Tuggle, S. Craig; Lavin, Kaleen M.; Bamman, Marcas M.; McAdam, Jeremy S.; Graham, Zachary A.; Peoples, Brandon; Seay, Regina S.; Lavin, Kaleen M.; Gargus, Amber B.; Yang, Sufen; O’Bryan, Samia M.; Drummer, Devin J.; Kelley, Christian J.; Bell, Margaret B.; Aban, Inmaculada; Cutter, Gary R.; Bamman, Marcas M.; Craig, Michael P.; Zhang, Jin; Hira, Akshay; Broderick, Timothy J.; Kadakia, Madhavi P.; Graham, Zachary A.; Peri, Kalyani; Bell, Margaret B.; and Aban, Inmaculada, "Multidimensional biocircuitry of exercise adaptation" (2025). Physiology Faculty Publications. 240.
https://uknowledge.uky.edu/physiology_facpub/240