Author ORCID Identifier

https://orcid.org/0000-0002-9086-8588

Year of Publication

2019

Degree Name

Master of Science (MS)

Document Type

Master's Thesis

College

Medicine

Department

Medical Sciences

First Advisor

Dr. Jerold Woodward

Abstract

The discovery that cationic lipids can independently stimulate the immune system has generated interest in their potential as vaccine adjuvants. Here, we show that the cationic lipid R-DOTAP can independently stimulate type 1 interferon production in dendritic cells in both primary culture and immortalized cell culture. Levels of type 1 interferon production are cell line-dependent and limited in vitro by lipid-induced cell death. We show that cationic lipids can independently activate TLR-7 and TLR-9, suggesting a mechanism for type 1 interferon induction. This TLR-stimulatory activity is not restricted to R-DOTAP and can be extended to other similar cationic lipids in a lipid-specific and TLR-specific manner.

Digital Object Identifier (DOI)

https://doi.org/10.13023/etd.2019.145

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