Author ORCID Identifier
Date Available
4-26-2019
Year of Publication
2019
Degree Name
Master of Science (MS)
Document Type
Master's Thesis
College
Medicine
Department/School/Program
Medical Sciences
First Advisor
Dr. Jerold Woodward
Abstract
The discovery that cationic lipids can independently stimulate the immune system has generated interest in their potential as vaccine adjuvants. Here, we show that the cationic lipid R-DOTAP can independently stimulate type 1 interferon production in dendritic cells in both primary culture and immortalized cell culture. Levels of type 1 interferon production are cell line-dependent and limited in vitro by lipid-induced cell death. We show that cationic lipids can independently activate TLR-7 and TLR-9, suggesting a mechanism for type 1 interferon induction. This TLR-stimulatory activity is not restricted to R-DOTAP and can be extended to other similar cationic lipids in a lipid-specific and TLR-specific manner.
Digital Object Identifier (DOI)
https://doi.org/10.13023/etd.2019.145
Recommended Citation
Bush, John Peyton, "The Stimulation of Dendritic Cells by Cationic Lipids" (2019). Theses and Dissertations--Medical Sciences. 10.
https://uknowledge.uky.edu/medsci_etds/10
