Abstract
CD8þ T cells from HLA-A2.1-transgenic mice, but not wild-type mice, immunized with the amino- terminus region (aa 41e152) of dense granule protein 6 (GRA6Nt) of Toxoplasma gondii secreted large amounts of perforin and granzyme B in response to GRA6Nt through antigen presentation by HLA-A2.1 in vitro. When those CD8þ T cells were transferred into chronically infected HLA-A2.1-expressing NSG mice deficient in T cells, cerebral cyst burden of the recipients of HLA-A2.1-transgenic T cells, but not of WT T cells, became significantly less than that of control mice with no cell transfer. Furthermore, the significant reduction of the cyst burden by a transfer of the HLA-A2.1-transgenic CD8þ immune T cells required an expression of HLA-A2.1 in the recipient NSG mice. Thus, antigen presentation of GRA6Nt by human HLA-A2.1is able to activate anti-cyst CD8þ T cells that eliminate T. gondii cysts through antigen presentation by human HLA-A2.1.
Document Type
Article
Publication Date
12-2023
Digital Object Identifier (DOI)
https://doi.org/10.1016/j.micinf.2023.105182
Funding Information
The studies are supported in part by the National Institutes of Health, the United States (NIH) grants (AI095032, AI152597, AI136821, and AI134323) (Y.S.). The authors appreciate the support from the Protein Core Laboratory of the University of Kentucky College of Medicine, United States for production and purification of rGRA6Nt.
Repository Citation
Mani, Rajesh; Mercier, Corinne; Delauw, Marie-France; and Suzuki, Yasuhiro, "Immunization with the amino-terminus region of dense granule protein 6 (GRA6) of Toxoplasma gondii activates CD8+ cytotoxic T cells capable of removing tissue cysts of the parasite through antigen presentation by human HLA-A2.1" (2023). Markey Cancer Center Faculty Publications. 345.
https://uknowledge.uky.edu/markey_facpub/345
Notes/Citation Information
© 2023 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.