RNA-Sequencing Reveals Global Transcriptomic Changes in Nicotiana tabacum Responding to Topping and Treatment of Axillary-Shoot Control Chemicals

Authors

Sanjay Kumar Singh, University of KentuckyFollow
Yongmei Wu, University of KentuckyFollow
Jayadri S. Ghosh, University of KentuckyFollow
Sitakanta Pattanaik, University of KentuckyFollow
Colin Fisher, University of KentuckyFollow
Ying Wang, Chinese Academy of Sciences, China
Darlene Lawson, R. J. Reynolds
Ling Yuan, University of KentuckyFollow

Abstract

Removal of terminal buds (topping) and control of the formation of axillary shoots (suckers) are common agronomic practices that significantly impact the yield and quality of various crop plants. Application of chemicals (suckercides) to plants following topping is an effective method for sucker control. However, our current knowledge of the influence of topping, and subsequent suckercide applications, to gene expression is limited. We analyzed the differential gene expression using RNA-sequencing in tobacco (Nicotiana tabacum) that are topped, or treated after topping by two different suckercides, the contact-localized-systemic, Flupro^® (FP), and contact, Off-Shoot-T^®. Among the differentially expressed genes (DEGs), 179 were identified as common to all three conditions. DEGs, largely related to wounding, phytohormone metabolism and secondary metabolite biosynthesis, exhibited significant upregulation following topping, and downregulation after suckercide treatments. DEGs related to photosynthetic processes were repressed following topping and suckercide treatments. Moreover, topping and FP-treatment affect the expression of auxin and cytokinin signaling pathway genes that are possibly involved in axillary shoot formation. Our results provide insights into the global change of plant gene expression in response to topping and suckercide treatments. The regulatory elements of topping-inducible genes are potentially useful for the development of a chemical-free sucker control system.

Document Type

Article

Publication Date

12-16-2015

Notes/Citation Information

Published in Scientific Reports, v. 5, article 18148, p. 1-13.

Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

Digital Object Identifier (DOI)

http://dx.doi.org/10.1038/srep18148

Repository Citation

Singh, Sanjay Kumar; Wu, Yongmei; Ghosh, Jayadri S.; Pattanaik, Sitakanta; Fisher, Colin; Wang, Ying; Lawson, Darlene; and Yuan, Ling, "RNA-Sequencing Reveals Global Transcriptomic Changes in Nicotiana tabacum Responding to Topping and Treatment of Axillary-Shoot Control Chemicals" (2015). Kentucky Tobacco Research and Development Center Faculty Publications. 13.
https://uknowledge.uky.edu/ktrdc_facpub/13