Theme 1: Grassland Ecology
Description
Endophytic phosphate-solubilizing bacteria from root nodules could promote stress resistance, nodulation and nitrogen fixation. In this study, nodule endophytic bacteria of Lespedeza daurica were isolated by plate streaking, the bacteria with phosphate-solubilizing abilities were selected and the phosphate-solubilizing abilities were determined. The results showed that 88 endophytic bacterial strains were isolated and among which 11 strains possessed phosphate-solubilizing abilities. The organic phosphorus solubility of TG41, TG43, TG47 and TG68 were 0.73~0.86 μg·mL-1 ; the inorganic phosphorus solubility of TG47 and TG68 were 72.97 and 1.29 μg·mL-1 , respectively. The four strains were identified as effective phosphate-solubilizing bacteria. TG41 and TG43 were determined as Bacillus zanthoxyli, TG47 as Phyllobacterium zundukense and TG68 as Pantoea agglomerans. There were abundant endophytic bacteria in root nodules of L. daurica and some of them possessed phosphate-solubilizing abilities, which could be used to research and develop composite microbial inoculants for rhizobia suitable for planting L. daurica in the Loess Plateau, China.
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Identification and Characteristics of Phosphate-solubilizing Endophytic Bacteria from Root Nodules of Lespedeza daurica
Endophytic phosphate-solubilizing bacteria from root nodules could promote stress resistance, nodulation and nitrogen fixation. In this study, nodule endophytic bacteria of Lespedeza daurica were isolated by plate streaking, the bacteria with phosphate-solubilizing abilities were selected and the phosphate-solubilizing abilities were determined. The results showed that 88 endophytic bacterial strains were isolated and among which 11 strains possessed phosphate-solubilizing abilities. The organic phosphorus solubility of TG41, TG43, TG47 and TG68 were 0.73~0.86 μg·mL-1 ; the inorganic phosphorus solubility of TG47 and TG68 were 72.97 and 1.29 μg·mL-1 , respectively. The four strains were identified as effective phosphate-solubilizing bacteria. TG41 and TG43 were determined as Bacillus zanthoxyli, TG47 as Phyllobacterium zundukense and TG68 as Pantoea agglomerans. There were abundant endophytic bacteria in root nodules of L. daurica and some of them possessed phosphate-solubilizing abilities, which could be used to research and develop composite microbial inoculants for rhizobia suitable for planting L. daurica in the Loess Plateau, China.
