Track 4-1-4: Biotechnological Approaches to Improve Range, Pasture and Forage Species
Description
Sehima nervosum is one of the important rangeland grass in India, It is commonly known as Saen grass in India, white grass in Australia, and has also been reported from the Central East Africa and Sudan. It is a good forage grass and maybe utilized for grazing as well as for hay preparation. It is a perennial grass, prefers hot and dry climate and survive even in limited rainfalls. As this natural grass is found inherently rich in precursors for several industrially important biomolecules, fractionation of these precursors seems to be a promising endeavour. Production of nutraceuticals (prebiotic xylo-oligosaccharides) from the lignocellulosic biomass of this grass is promising, as this grass does not compete with food crops, and is comparatively less expensive than conventional agricultural food-stocks. However, germplasm of this grass has narrow genetic variability. Being largely apomictic in reproduction, generation of variability through hybridization approaches have been limited. Utilization of biotechnological tools is one of the potential ways for introducing variability and transfer of desirable traits. The development of an efficient genetic transformation procedure for Sehima could facilitate physiological and molecular biology studies as well as the production of transgenic cultivars for higher productivity and quality.
To the best of our knowledge there are no reports on in vitro callus induction, regeneration and transformation in Sehima. Herein, for the first time, efficient in vitro callus induction from mature seed explant and transformation efficiency in Sehima is reported. Here we standardized a reproducible, rapid and efficient Agrobacterium mediated transformation using Agrobacterium strain EHA105 harbouring binary vector pCAMBIA 1305.
Citation
Dwivedi, Krishna Kumar; Sonkar, Tanu; Katiyar, R.; Radhakrishna, Auji; and Kaushal, P., "Establishment of High-Efficiency Agrobacterium-Mediated Transformation of Callus Derived from Sehima nervosum, an Important Range Grass Species" (2020). IGC Proceedings (1993-2023). 10.
https://uknowledge.uky.edu/igc/23/4-1-4/10
Included in
Establishment of High-Efficiency Agrobacterium-Mediated Transformation of Callus Derived from Sehima nervosum, an Important Range Grass Species
Sehima nervosum is one of the important rangeland grass in India, It is commonly known as Saen grass in India, white grass in Australia, and has also been reported from the Central East Africa and Sudan. It is a good forage grass and maybe utilized for grazing as well as for hay preparation. It is a perennial grass, prefers hot and dry climate and survive even in limited rainfalls. As this natural grass is found inherently rich in precursors for several industrially important biomolecules, fractionation of these precursors seems to be a promising endeavour. Production of nutraceuticals (prebiotic xylo-oligosaccharides) from the lignocellulosic biomass of this grass is promising, as this grass does not compete with food crops, and is comparatively less expensive than conventional agricultural food-stocks. However, germplasm of this grass has narrow genetic variability. Being largely apomictic in reproduction, generation of variability through hybridization approaches have been limited. Utilization of biotechnological tools is one of the potential ways for introducing variability and transfer of desirable traits. The development of an efficient genetic transformation procedure for Sehima could facilitate physiological and molecular biology studies as well as the production of transgenic cultivars for higher productivity and quality.
To the best of our knowledge there are no reports on in vitro callus induction, regeneration and transformation in Sehima. Herein, for the first time, efficient in vitro callus induction from mature seed explant and transformation efficiency in Sehima is reported. Here we standardized a reproducible, rapid and efficient Agrobacterium mediated transformation using Agrobacterium strain EHA105 harbouring binary vector pCAMBIA 1305.
