Satellite Symposium 5: Molecular Breeding
Description
The genomes of many plants are known to be composed of a large fraction of repetitive DNA, while a small portion is dedicated to genes. The bulk of the repetitive DNA constitutes transposable elements and is heavily methylated. GeneThresher technology has been developed to take advantage of these differential methylation patterns by filtering genomic shotgun libraries to exclude methylated sequences (Rabinowicz et al., 1999; Palmer et al., 2003; Martienssen et al., 2004). The result is a gene-enriched genomic shotgun library. Random shotgun sequencing of plant gene space, enabled by GeneThresher technology, is a rapid and cost-effective strategy for comprehensive gene discovery in agriculturally important crops
Citation
Warek, U.; Bedell, J. A.; Budiman, M. A.; Nunberg, A. N.; Citek, R. W.; Robbins, D.; Lakey, N.; and Rabinowicz, P. D., "The Efficacy of GeneThresher® Methylation Filtering Technology in the Plant Kingdom" (2023). IGC Proceedings (1993-2023). 3.
https://uknowledge.uky.edu/igc/20/satellitesymposium5/3
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
The Efficacy of GeneThresher® Methylation Filtering Technology in the Plant Kingdom
The genomes of many plants are known to be composed of a large fraction of repetitive DNA, while a small portion is dedicated to genes. The bulk of the repetitive DNA constitutes transposable elements and is heavily methylated. GeneThresher technology has been developed to take advantage of these differential methylation patterns by filtering genomic shotgun libraries to exclude methylated sequences (Rabinowicz et al., 1999; Palmer et al., 2003; Martienssen et al., 2004). The result is a gene-enriched genomic shotgun library. Random shotgun sequencing of plant gene space, enabled by GeneThresher technology, is a rapid and cost-effective strategy for comprehensive gene discovery in agriculturally important crops
