Satellite Symposium 5: Molecular Breeding

Description

The Brazilian cattle herd comprises 185 million animals fed with about 177 million hectares of native and cultivated pastures (IBGE, 2002). Of the grass species used for forage in Brazil, the African genus Brachiaria is the most widely planted, followed by Panicum, which also has an African origin. Legumes of the Stylosanthes genus, native to the South America, have emerged in the last few years as potential forage species for use with the grasses. These forage species have been bred at Embrapa Beef Cattle and the breeding programs have shown the need for more genetic information including the use of molecular markers. The objectives of this work were to construct microsatellite-enriched genomic libraries for 5 species of Brachiaria (B. brizantha, B. decumbens, B. dictioneura, B. humidicola and B. ruziziensis), for P. maximum and for S. capitata, and to characterize the microsatellites found in B. brizantha.

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Construction of Microsatellite-Enriched Libraries for Tropical Forage Species and Characterization of the Repetitive Sequences Found in Brachiaria Brizantha

The Brazilian cattle herd comprises 185 million animals fed with about 177 million hectares of native and cultivated pastures (IBGE, 2002). Of the grass species used for forage in Brazil, the African genus Brachiaria is the most widely planted, followed by Panicum, which also has an African origin. Legumes of the Stylosanthes genus, native to the South America, have emerged in the last few years as potential forage species for use with the grasses. These forage species have been bred at Embrapa Beef Cattle and the breeding programs have shown the need for more genetic information including the use of molecular markers. The objectives of this work were to construct microsatellite-enriched genomic libraries for 5 species of Brachiaria (B. brizantha, B. decumbens, B. dictioneura, B. humidicola and B. ruziziensis), for P. maximum and for S. capitata, and to characterize the microsatellites found in B. brizantha.