Production and Analysis of Transgenic White Clover (Trifolium Repens) Plants Over-Expressing Organic Acid Biosynthetic Genes

Presenter Information

C. M. Labandera, Molecular Plant Breeding Cooperative Research Centre, AustraliaFollow
Stephen N. Panter, Molecular Plant Breeding Cooperative Research Centre, AustraliaFollow
A. Winkworth, La Trobe University, Australia
J. Simmonds, La Trobe University, Australia
A. Mouradov, La Trobe University, Australia
U. John, La Trobe University, Australia
P. W. Sale, La Trobe University, Australia
G. C. Spangenberg, La Trobe University, AustraliaFollow

Description

Aluminium (Al) toxicity is a major environmental limitation for plant production in acid soils, which represent more than one third of the world’s agricultural land. Al-induced secretion in roots of organic acids (OA), such as malate and citrate, chelates the toxic Al cation excluding it from the root. This mechanism of Al-tolerance appears also to be associated with enhanced P-use efficiency. The development of transgenic plants for enhanced synthesis and secretion of OA from roots is a promising approach to confer Al-tolerance and enhanced P-acquisition efficiency. In order to understand the association between OA biosynthesis and secretion from roots in white clover (Trifolium repens L.), the physiological consequences of over-expressing 3 key white clover OA biosynthetic genes, individually and in combination, were assessed in transgenic plants.

Citation

Labandera, C. M.; Panter, Stephen N.; Winkworth, A.; Simmonds, J.; Mouradov, A.; John, U.; Sale, P. W.; and Spangenberg, G. C., "Production and Analysis of Transgenic White Clover (Trifolium Repens) Plants Over-Expressing Organic Acid Biosynthetic Genes" (2023). IGC Proceedings (1993-2023). 132.
https://uknowledge.uky.edu/igc/20/satellitesymposium5/132