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Improving livestock efficiency in utilisation of nitrogen resources continues to be a major environmental and economic objective. Zhu et al. (1999) have shown that plant endopeptidases are activated as a response to cutting stress. Previous work in our laboratory explored over 300 entries of forage genotypes and found a broad diversity in enzymatic activity by means of hydrolysis in gelatine and direct autolysis assays in forage tissues. The objective of this work was to assess if the species previously identified as having high or low endopeptidase activity, would behave consistently when exposed to ruminal microbial proteolysis.

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Ruminal Proteolysis in Forages with Distinct Endopeptidases Activities

Improving livestock efficiency in utilisation of nitrogen resources continues to be a major environmental and economic objective. Zhu et al. (1999) have shown that plant endopeptidases are activated as a response to cutting stress. Previous work in our laboratory explored over 300 entries of forage genotypes and found a broad diversity in enzymatic activity by means of hydrolysis in gelatine and direct autolysis assays in forage tissues. The objective of this work was to assess if the species previously identified as having high or low endopeptidase activity, would behave consistently when exposed to ruminal microbial proteolysis.