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Coupling ruminal processes of hydrolysis and synthesis continues to be a research issue where more progress is needed. This requires the development of good protein assessment methods, particularly when representing the breakdown processes that occur in fresh pastures eaten by herbivores. Laboratory analyses need to deal with small and homogeneous samples, but the mechanical reduction of particle size may not reflect the actual digestion kinetics occurring when the original fresh forage is consumed. Such physical traits may alter the release of non-structural compounds and the penetration of microbial enzymes (Boudon et al., 2002). The objective of this work was to assess in fresh samples the effect of reducing particle size upon the in vitro breakdown of proteins during the early rumen fermentation period.

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Effects of Particle Size in Forage Samples for Protein Breakdown Studies

Coupling ruminal processes of hydrolysis and synthesis continues to be a research issue where more progress is needed. This requires the development of good protein assessment methods, particularly when representing the breakdown processes that occur in fresh pastures eaten by herbivores. Laboratory analyses need to deal with small and homogeneous samples, but the mechanical reduction of particle size may not reflect the actual digestion kinetics occurring when the original fresh forage is consumed. Such physical traits may alter the release of non-structural compounds and the penetration of microbial enzymes (Boudon et al., 2002). The objective of this work was to assess in fresh samples the effect of reducing particle size upon the in vitro breakdown of proteins during the early rumen fermentation period.