Satellite Symposium 2: Silage
Description
Ensilage of herbaceous biomass can be enhanced by applying pre-selected fermentative bacteria, however insufficient is known about the population dynamics of such starter cultures under a range of ensiling conditions. Classical methods for species-specific quantification of bacteria are labour intensive. An alternative approach is the detection of bacteria based on molecular markers for species-specific regions within their genomic DNA (e.g. the 16S rDNA sequence). In this study, a quantitative marker assay using the real-time PCR technique (Q-PCR) is described for Lactobacillus plantarum, a bacterium often used for silage starter cultures.
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
A 16S rDNA-Based Quantitative Assay for Monitoring Lactobacillus Plantarum in Silage
Ensilage of herbaceous biomass can be enhanced by applying pre-selected fermentative bacteria, however insufficient is known about the population dynamics of such starter cultures under a range of ensiling conditions. Classical methods for species-specific quantification of bacteria are labour intensive. An alternative approach is the detection of bacteria based on molecular markers for species-specific regions within their genomic DNA (e.g. the 16S rDNA sequence). In this study, a quantitative marker assay using the real-time PCR technique (Q-PCR) is described for Lactobacillus plantarum, a bacterium often used for silage starter cultures.
