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Publication Date
1997
Location
Manitoba and Saskatchewan
Description
An isogenic population of sainfoin (Onobrychis viciifolia Scop.) plants was developed by two methods; regeneration after long term tissue culture and plant transformation with an antisense chalcone synthase gene. Tannin (proanthocyanidin) content could be dramatically increased in untransformed callus by long term culture, but was not significantly altered in regenerating plantlets. Some of the mature transgenic plants had dramatically reduced levels of leaf tannin. The antisense gene was eliminated from all transgenic plants later in development.
Citation
Gruber, M Y.; Auser, P; Lees, G; Muir, A D.; and Wood, M, "Genetic Manipulation of Sainfoin Leaf Tannins" (1997). IGC Proceedings (1985-2023). 4.
(URL: https://uknowledge.uky.edu/igc/1997/session8/4)
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Genetic Manipulation of Sainfoin Leaf Tannins
Manitoba and Saskatchewan
An isogenic population of sainfoin (Onobrychis viciifolia Scop.) plants was developed by two methods; regeneration after long term tissue culture and plant transformation with an antisense chalcone synthase gene. Tannin (proanthocyanidin) content could be dramatically increased in untransformed callus by long term culture, but was not significantly altered in regenerating plantlets. Some of the mature transgenic plants had dramatically reduced levels of leaf tannin. The antisense gene was eliminated from all transgenic plants later in development.
