Description
A rapid screening technique was developed for use in evaluating white clover (Trifolium repens L.) for resistance to Cylindrocladium root rot incited by Cylindrocladium crotalariae. Culture plates of the fungus were evaluated for number of infective propagules after four weeks of growth on potato dextrose agar, and no differences were detected among plates. Seedlings of ‘Osceola’ white clover were exposed in the greenhouse to four levels of inoculum over a six-week period, with resistance based on percent survival of uninoculated controls. It was shown that suspending 1 culture plate in 100ml of water and rating plants four weeks after inoculation was suitable for screening seedlings. A field test was conducted to verify greenhouse results. Results indicated that the greenhouse technique gave similar results, and would be sufficient to use in a breeding program to develop resistance to this disease.
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Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Evaluating White Clover for Resistance to Cylindrocladium Root Rot
A rapid screening technique was developed for use in evaluating white clover (Trifolium repens L.) for resistance to Cylindrocladium root rot incited by Cylindrocladium crotalariae. Culture plates of the fungus were evaluated for number of infective propagules after four weeks of growth on potato dextrose agar, and no differences were detected among plates. Seedlings of ‘Osceola’ white clover were exposed in the greenhouse to four levels of inoculum over a six-week period, with resistance based on percent survival of uninoculated controls. It was shown that suspending 1 culture plate in 100ml of water and rating plants four weeks after inoculation was suitable for screening seedlings. A field test was conducted to verify greenhouse results. Results indicated that the greenhouse technique gave similar results, and would be sufficient to use in a breeding program to develop resistance to this disease.
