Publication Date
1997
Description
NIR calibration equations were developed to determine crude protein (CP), acid detergent fibre (ADF), organic matter (OM), in vivo organic matter digestibility (OMD) and metabolizable energy (ME) in mixed swards silages. Spectra of 96 in vivo evaluated silages were recorded on a PSCO 6250 monochromator spectrophotometer using NSAS (NIRS Spectral Analysis Software) and converted to NewISI software. NIR calibrations were obtained by modified PLS regression of the 2nd. derivative of SNV and De-trended spectra on laboratory and in vivo data. Determination coefficients (R2) ranged from 0.77 for ME to 0.98 for CP and OM. Highest standard errors of calibration and cross-validation were respectively 2.20 and 2.40 for OMD and lowest were 0.43 and 0.78 for CP. A set of 199 silages from commercial farms was used to validate OM, CP and ADF data. CP was the most precise of these determinations (R2= 0.90, SEP= 1.20).
Citation
Castro, P; Castro, J; Gonzalez-Arraez, A; and Flores, G, "Analysis of Mixed Sward Silage by Near Infrared Reflectance Spectroscopy (NIRS)" (2024). IGC Proceedings (1993-2023). 38.
https://uknowledge.uky.edu/igc/1997/session17/38
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Analysis of Mixed Sward Silage by Near Infrared Reflectance Spectroscopy (NIRS)
NIR calibration equations were developed to determine crude protein (CP), acid detergent fibre (ADF), organic matter (OM), in vivo organic matter digestibility (OMD) and metabolizable energy (ME) in mixed swards silages. Spectra of 96 in vivo evaluated silages were recorded on a PSCO 6250 monochromator spectrophotometer using NSAS (NIRS Spectral Analysis Software) and converted to NewISI software. NIR calibrations were obtained by modified PLS regression of the 2nd. derivative of SNV and De-trended spectra on laboratory and in vivo data. Determination coefficients (R2) ranged from 0.77 for ME to 0.98 for CP and OM. Highest standard errors of calibration and cross-validation were respectively 2.20 and 2.40 for OMD and lowest were 0.43 and 0.78 for CP. A set of 199 silages from commercial farms was used to validate OM, CP and ADF data. CP was the most precise of these determinations (R2= 0.90, SEP= 1.20).
