Publication Date

1993

Description

Variations in isoflavone and pterocarpan contents were studied in cloned material of red clover (Trifolium pratense L,), The most abundant constituents in the leaves, as determined by gas chromatography, were formononetin (50%) and biochanin A (25%), whereas the rools were characterised by the pterocarpan manckiain (45%). The amounts of leaf isoflavones (range 100-880 µg/100 mg DM) differed significantly between clones at subsequent growth stages, and considerably lower levels were found when compared with the control material. The contents were highest in the beginning of the growing season (June), but decreased strongly towards the end of the lowering period. The results show a considerable genetic variation of isoflavones which can be utilised in the breeding of red clover varieties with lower isoflavone levels.

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Variation in the Isoflavone and Pterocarpan Contents of Red Clover

Variations in isoflavone and pterocarpan contents were studied in cloned material of red clover (Trifolium pratense L,), The most abundant constituents in the leaves, as determined by gas chromatography, were formononetin (50%) and biochanin A (25%), whereas the rools were characterised by the pterocarpan manckiain (45%). The amounts of leaf isoflavones (range 100-880 µg/100 mg DM) differed significantly between clones at subsequent growth stages, and considerably lower levels were found when compared with the control material. The contents were highest in the beginning of the growing season (June), but decreased strongly towards the end of the lowering period. The results show a considerable genetic variation of isoflavones which can be utilised in the breeding of red clover varieties with lower isoflavone levels.