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Publication Date

1993

Location

New Zealand

Description

A cellulase cDNA, ce/E, was isolated from a cellulose-induced cDNA library of the rumen fungus Neocallimastix patriciarum by screening for hydrolysis of carboxymethyl cellulose. In addition to its ability to hydrolyse carboxymethyl cellulose, ce/E enzyme was also able to degrade microcrystalline cellulose, methylumbelliferyl cellobioside, p-nitrophenyl cellobioside, lichenan and xylan. These data suggest that ce/E encodes a multi-functional cellulase.

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Isolation of a Multi-Functional Cellulase cDNA (celE) from the Rumen Fungus Neocallimastix patriciarum

New Zealand

A cellulase cDNA, ce/E, was isolated from a cellulose-induced cDNA library of the rumen fungus Neocallimastix patriciarum by screening for hydrolysis of carboxymethyl cellulose. In addition to its ability to hydrolyse carboxymethyl cellulose, ce/E enzyme was also able to degrade microcrystalline cellulose, methylumbelliferyl cellobioside, p-nitrophenyl cellobioside, lichenan and xylan. These data suggest that ce/E encodes a multi-functional cellulase.