Application of Ovule Culture to Interspecific Hybridization Between Trifolium repens and T. Ambiguum
Publication Date
1985
Location
Kyoto Japan
Description
The objective of the present study was to apply the ovule culture for the production of interspecific hybrids between Trifolium repens and T. ambiguum. Significant ovary development occurred only when T. ambiguum was used as the female parent. After interspecific fertilization, almost all hybrid ovules fail to develop from about 7th day after pollination. Observation of the growth pattern of hybrid ovules suggests that these should be excised and transplanted to the culture medium by the 5th day after pollination, A medium containing minerals of the minor modified Nitsch (1951), Vitamines (Murashige and Skoog 1962), 7% sucrose, 0.2% Casein hydrolysate and juice of young cucumber fruit was suitable for the culture of young hybrid ovules. After ovules had been inoculated with this medium for 15-20 days they were subcultured to a medium with 1 % sucrose and without cucumber juice, then some of these ovules germinated. Thus hybrid plants were efficiently obtained through ovule culture.
Citation
Yamada, T and Fukoka, H, "Application of Ovule Culture to Interspecific Hybridization Between Trifolium repens and T. Ambiguum" (1985). IGC Proceedings (1985-2023). 19.
(URL: https://uknowledge.uky.edu/igc/1985/ses2/19)
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Pathology Commons, Soil Science Commons, Weed Science Commons
Application of Ovule Culture to Interspecific Hybridization Between Trifolium repens and T. Ambiguum
Kyoto Japan
The objective of the present study was to apply the ovule culture for the production of interspecific hybrids between Trifolium repens and T. ambiguum. Significant ovary development occurred only when T. ambiguum was used as the female parent. After interspecific fertilization, almost all hybrid ovules fail to develop from about 7th day after pollination. Observation of the growth pattern of hybrid ovules suggests that these should be excised and transplanted to the culture medium by the 5th day after pollination, A medium containing minerals of the minor modified Nitsch (1951), Vitamines (Murashige and Skoog 1962), 7% sucrose, 0.2% Casein hydrolysate and juice of young cucumber fruit was suitable for the culture of young hybrid ovules. After ovules had been inoculated with this medium for 15-20 days they were subcultured to a medium with 1 % sucrose and without cucumber juice, then some of these ovules germinated. Thus hybrid plants were efficiently obtained through ovule culture.
